A comparison of the vasodilatory effects of verapamil, papaverine and nitroglycerin on isolated rat aorta
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Baycinar Medical Publ-Baycinar Tibbi Yayincilik
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: This study aims to compare the vasodilatory effects of verapamil, papaverine and nitroglycerin on rat aortic preparations in in vitro isolated tissue bath system and to evaluate the role of vascular endothelium on vasodilatory responses of the isolated rat aorta samples. Methods: The thoracic aorta segments collected from 30 male Wistar rats (20 endothelialized and 20 de-endothelialized vascular rings for each drug in 2 mm wide strips, total number of 120 vascular rings) were suspended into the Krebs solution of the isolated tissue bath system. Phenylephrine was used to induce isometric contraction and tissue samples were treated with verapamil, papaverine and nitroglycerin separately to draw concentration-response curves of isometric vasodilatory responses. This procedure was repeated for de-endothelialized aorta samples. Results: Papaverine and verapamil induced vasodilatatory responses starting from the concentration of 10(-8) M and reached its maximum at concentration of 10(-3) M, while nitroglycerin induced vasodilation at lower concentrations starting from a concentration of 10(-12) M, reaching its maximum at 10(-6) M. Nitroglycerin was the most potent agent, followed by verapamil and papaverine. Efficacy analysis revealed that the most efficient agents were papaverine (140 +/- 6.7%), nitroglycerin (110.8 +/- 1.35%) and verapamil (99 +/- 4.14%), respectively. The results were similar in aorta samples without endothelium (p >= 0.05, F test). Conclusion: In this study examining isolated rat aorta, nitroglycerin was the most potent agent, while papaverine was the most efficient agent. Our study results showed that endothelium played no role in vasodilatation responses of these drugs.
Açıklama
Anahtar Kelimeler
Aorta, Nitroglycerin, Papaverine, Vasodilatation, Verapamil, Internal Thoracic Artery, Rabbit Aorta, Relaxation, Endothelium, Phenoxybenzamine, Tolerance, Responses, Nitrate
Kaynak
Turk Gogus Kalp Damar Cerrahisi Dergisi-Turkish Journal Of Thoracic And Cardiovascular Surgery
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
21
Sayı
4
