The Expression Profiles of Angio-miRs in Glioblastomas Invasion Inhibited by Ruxolitinib

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dc.contributor.authorDelen, Emre
dc.contributor.authorDoganlar, Oguzhan
dc.date.accessioned2024-06-12T11:08:39Z
dc.date.available2024-06-12T11:08:39Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAim: MicroRNAs (miR) have an essential role on the regulated gene expression in the human genome. In recent years, a specific miR group was called to angio-miRs due to their role in the angiogenesis, and recent study showed that they involved in the pathogenesis of gliomas. In this study, we investigated the changes in the expression profiles of angio-miRs in glioblastoma cells and identified relationship between these genes and invasion and tumor growth. Materials and Methods: In this study, glioblastoma tumor spheroids were obtained using the human glioblastoma cell line U-87 MG. 50 nM, 100 nM and 200 nM ruxolitinib were applied to tumor spheroids for 48 hours by using Matrigell method. Tumor volume and invasion formation relative olio tumor growth and relative olio invasion area were measured in glioblastoma tumor spheroids after 48 hours of treatment. At the same time, quantitative real-time polymerase chain reaction (qRT-PZR) analysis was performed and miR expression profiles were determined. The most important (importance features) miRNAs selected along with the heatmap and volcano plot analyzes were used to display the pattern of the differentially expressed miRs using normalized miR expression profiles. Results: When the effect of 50 nM, 100 nM and 200 nM ruxolitinib administration to tumor spheroids on tumor volume and invasion was evaluated, a significant difference was found at each dose applied. However, at the dose of 200 nM ruxolitinib, it was observed that the inhibitory effect of tumor invasion was the highest. When miR expression profiles obtained by qRT-PZR test with 200 nM ruxolitinib adminisration were evaluated, it was determined that the expression profiles of 10 miRs increased and the expression profiles of 4 miRs decreased. Conclusion: In conclusion, angio-miR expression profiles are important because they enable us to better understand the prognostic process of gliomas. Because of their multiple silencing properties, they may contribute to the clinic with further studies in terms of their use as new therapeutic targets and prognostic biomarkers for glioblastoma.en_US
dc.identifier.doi10.4274/nkmj.galenos.2021.25238
dc.identifier.endpage23en_US
dc.identifier.issn2587-0262
dc.identifier.issue1en_US
dc.identifier.startpage15en_US
dc.identifier.trdizinid1123731en_US
dc.identifier.urihttps://doi.org/10.4274/nkmj.galenos.2021.25238
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1123731
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22491
dc.identifier.volume10en_US
dc.identifier.wosWOS:001207838400015en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofNamik Kemal Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMiren_US
dc.subjectGlioblastomaen_US
dc.subjectAngiogenesisen_US
dc.subjectInvasionen_US
dc.subjectUnfavorable Prognosisen_US
dc.subjectMalignant Progressionen_US
dc.subjectCancer Metastasisen_US
dc.subjectMir-17-92 Clusteren_US
dc.subjectTumor Invasionen_US
dc.subjectUp-Regulationen_US
dc.subjectMicrornasen_US
dc.subjectMigrationen_US
dc.subjectMir-31en_US
dc.subjectRadixinen_US
dc.titleThe Expression Profiles of Angio-miRs in Glioblastomas Invasion Inhibited by Ruxolitiniben_US
dc.typeArticleen_US

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