The use of DNA microsatellite loci for "Caretta Caretta" identification

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dc.authorscopusid49764099300
dc.authorscopusid57213020098
dc.authorscopusid14050711900
dc.authorscopusid14050163200
dc.authorscopusid49762698800
dc.authorscopusid14051389200
dc.authorscopusid6506461315
dc.contributor.authorUysal S.
dc.contributor.authorPetridis G.
dc.contributor.authorOzcan S.
dc.contributor.authorFaikoglu R.
dc.contributor.authorBarcak D.
dc.contributor.authorYukseloglu H.
dc.contributor.authorAbaci-Kalfoglou E.
dc.date.accessioned2024-06-12T10:25:14Z
dc.date.available2024-06-12T10:25:14Z
dc.date.issued2006
dc.description.abstractCaretta carettas, one of the members of Chelonidae family, live primarily in water, except the periods of their ovulation where they come out to the shores to lay their eggs. Following an incubation period of 50-60 days, the newborns return to sea water to continue their 10-12-year life. Studies using marking methodology of the animals have shown that females return to the same place for ovulation every 2-3 years. The development of molecular genetic studies gives us now opportunity to trace all these routes that Caretta carettas follow during their life cycle. One of the basic techniques that is used for identification in general is the polymorphic DNA microsatellite loci. These 2-4 base pair DNA segments are considered to be ideal for Caretta caretta identification also. In this study we tried to establish a protocol in order to identify both male and female carettas in tissue samples collected from animals in the Mediterranean shoreline in the southern Turkey once this shoreline is one of the main spots of them for ovulation. The sampling has been done from 89 locations (Dalyan, Dalaman, Fethiye, Patara, Kale, Kumluca, Belek, Kizilot, Demirtaş, Gazipaşa and Anamur) from 246 dead baby Caretta carettas. DNA was extracted using silica-based extraction technology from the tissue homogenates. Cc7 locus was selected for identification to be tested for its degree of polymorphic content and therefore power of discrimination in general. The methodology used is PCR, followed by polyacrilamide gel electrophoresis and silver staining. Copyright © Taylor & Francis Group, LLC.en_US
dc.description.sponsorshipIstanbul Üniversitesi: T-368en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University. Project No T-368.en_US
dc.identifier.doi10.1080/10934520600779273
dc.identifier.endpage1987en_US
dc.identifier.issn1093-4529
dc.identifier.issue9en_US
dc.identifier.pmid16849141en_US
dc.identifier.scopus2-s2.0-33746429968en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1981en_US
dc.identifier.urihttps://doi.org/10.1080/10934520600779273
dc.identifier.urihttps://hdl.handle.net/20.500.14551/16254
dc.identifier.volume41en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineeringen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaretta Caretta; Dna; Identification; Mediterranean Shore Line; Microsattelites; Turkeyen_US
dc.subjectCaretta Caretta; Identification; Mediterranean Shore Line; Microsattelites; Turkey; Dna; Electrophoresis; Environmental Engineering; Extraction; Genetic Engineering; Marine Biology; Tissue; Biodiversity; Microsatellite Dna; Polyacrylamide; Silicon Dioxide; Silver; Animal Tissue; Caretta Caretta; Conference Paper; Controlled Study; Dna Extraction; Female; Gene Locus; Male; Mediterranean Sea; Nonhuman; Ovulation; Polyacrylamide Gel Electrophoresis; Polymerase Chain Reaction; Silver Staining; Tissue Homogenate; Turtle; Animals; Behavior, Animal; Electrophoresis, Polyacrylamide Gel; Female; Male; Mediterranean Sea; Microsatellite Repeats; Polymerase Chain Reaction; Polymorphism, Genetic; Turkey; Turtles; Animalia; Caretta; Caretta Carettaen_US
dc.titleThe use of DNA microsatellite loci for "Caretta Caretta" identificationen_US
dc.typeConference Objecten_US

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