A simple immunosensor for thyroid stimulating hormone

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dc.authoridOZCAN, Hakki/0000-0003-2204-7267
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorAydin, Umut Deniz
dc.date.accessioned2024-06-12T11:16:26Z
dc.date.available2024-06-12T11:16:26Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractDetermination of thyroid-stimulating hormone (TSH) level in serum or plasma is defined as a sensitive method for the diagnosis of hyperthyroidism and hypothyroidism and also in many diseases thought to be related to TSH levels. In this study, a novel simple impedimetric immunosensor based on polyamidoamine dendrimer was developed. Anti TSH antibody was immobilized on the gold electrode by using cysteamine self-assembled monolayer strategy. In constructing the immunosensor, a polyamidoamine dendrimer was used to increase the surface area in which Anti-TSH was immobilized and glutaraldehyde was used as a cross-linker. After each immobilization step, the electrode surface was monitored by electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy and energy-dispersive X-ray spectroscopy techniques and optimization studies were performed. The reproducibility, repeatability, linearity and sensitivity of the immunosensor were examined. Also, the interference experiments for glucose, salts and proteins in serum were performed. The limit of detection and limit of quantification values of the proposed immunosensor were 0.026 mIUL(-1) and 0.086 mIUL(-1), respectively and it was able to detect the amount of TSH within a linear range of 0.1-0.6 mIUL(-1).en_US
dc.description.sponsorshipTrakya Universitesi Scientific Research Foundation [TUBAP - 2017/109]en_US
dc.description.sponsorshipThis study is supported by Trakya Universitesi Scientific Research Foundation (Project Number: TUBAP - 2017/109).en_US
dc.identifier.doi10.1080/21691401.2020.1867153
dc.identifier.endpage70en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue1en_US
dc.identifier.pmid33410369en_US
dc.identifier.scopus2-s2.0-85099186961en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage61en_US
dc.identifier.urihttps://doi.org/10.1080/21691401.2020.1867153
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24303
dc.identifier.volume49en_US
dc.identifier.wosWOS:000605724100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectThyroid-Stimulating Hormoneen_US
dc.subjectImmunosensoren_US
dc.subjectElectrochemical Impedance Spectroscopyen_US
dc.subjectSelf-Assembled Monolayeren_US
dc.subjectCyclic Voltammetryen_US
dc.titleA simple immunosensor for thyroid stimulating hormoneen_US
dc.typeArticleen_US

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