The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

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dc.contributor.authorYapar, Suleyman Bedir
dc.contributor.authorEskiocak, Sevgi
dc.date.accessioned2024-06-12T10:51:18Z
dc.date.available2024-06-12T10:51:18Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: It is known that oxidant species play a role in the pathogenesis of certain diseases such as diabetes mellitus, atherosclerosis, cataract and hepatic cirrhosis. Therefore, the use of antioxidant species for therapeutic purposes has risen up in the recent years. The aim of the study was to investigate the different concentrations of alpha lipoic acid on the induced lipid peroxidation and tissue glutathione level in rat liver homogenates. Methods: Hydrogen peroxide (15 mM) has been used for induction of lipid peroxidation at liver homogenates. The experimental setups induced by lipid peroxidation have been divided into four sub-groups. Alpha lipoic acid was added in 0, 2, 4 and 8 mM concentrations into those groups, respectively. The malondialdehyde levels which is the end product of lipid peroxidation and the levels of tissue glutathione have been determined. Results: The level of malondialdehyde in the activation groups has been found to be significantly higher than to the control group. The levels of malondialdehyde in the all alpha lipoic acid groups have been found to be significantly lower than the activation group. The level of glutathione in the activation group has been detected significantly lower when it was compared to the control group. The levels of glutathione in the all alpha lipoic acid groups have been found to be significantly higher from the activation group. When the time-dependent change in the level of glutathione was investigated it was observed that these initially decrease and then started to increase. In the groups of 4 and 8 mM, this level was even over from the starting point. Conclusion: In conclusion, our findings suggest that lipid peroxidation is induced in the experimental setups where hydrogen peroxide are applied. The reason of significantly lower malondialdehyde and higher glutathione levels in alpha lipoic acid group than activation groups may be a result of the antioxidant property of alpha lipoic acid.en_US
dc.identifier.doi10.5505/tjb.2014.71501
dc.identifier.endpage327en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84912026320en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage317en_US
dc.identifier.urihttps://doi.org/10.5505/tjb.2014.71501
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18307
dc.identifier.volume39en_US
dc.identifier.wosWOS:000346148200010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTurkish Biochem Socen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha Lipoic Aciden_US
dc.subjectMalondialdehydeen_US
dc.subjectGlutathioneen_US
dc.subjectHydrogen Peroxideen_US
dc.subjectInduced Oxidative Stressen_US
dc.subjectLens Epithelial-Cellsen_US
dc.subjectIn-Vitroen_US
dc.subjectFree-Radicalsen_US
dc.subjectAntioxidantsen_US
dc.subjectGlutathioneen_US
dc.subjectMelatoninen_US
dc.subjectRedoxen_US
dc.subjectIronen_US
dc.subjectTissuesen_US
dc.titleThe effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenatesen_US
dc.typeArticleen_US

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