Effects of sphingosylphosphorylcholine against cholestatic oxidative stress and liver damage in the common bile duct ligated rats

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dc.authoridUmit, Hasan/0000-0002-3651-4180
dc.authoridUzun, Hafize/0000-0002-1347-8498
dc.authorwosidAktas, Cevat/D-8468-2011
dc.authorwosidGuzel, Ahmet/AGE-2880-2022
dc.authorwosidUmit, Hasan/T-6236-2019
dc.authorwosidUzun, Hafize/D-4811-2019
dc.contributor.authorAksu, Burhan
dc.contributor.authorUmit, Hasan
dc.contributor.authorKanter, Mehmet
dc.contributor.authorGuzel, Ahmet
dc.contributor.authorInan, Mustafa
dc.contributor.authorCivelek, Sabiha
dc.contributor.authorAktas, Cevat
dc.date.accessioned2024-06-12T11:15:26Z
dc.date.available2024-06-12T11:15:26Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe goal of this study was to evaluate the possible protective effects of sphingosylphosphorylcholine (SPC) against cholestatic oxidative stress and liver damage in the common bile duct ligated rats. Fifty-six animals were included in each of the following 7 groups: control, SPC control, phosphate-buffered solution control, sham operated, bile duct ligation (BDL), BDL plus phosphate-buffered solution, and BDL plus SPC. Sphingosylphosphorylcholine was administered 14 days at a daily dose of 2 mu m/mL intraperitoneally. The severity of cholestasis and hepatic injury was determined by changes in the plasma enzyme activities of aspartate aminotransferase, alanine aminotransferase, gama glutamin transferase, and levels of total bilirubin and direct bilirubin. Malondialdehyde, nitric oxide, and superoxide dismutase were determined to evaluate the oxidative status in the liver tissue. Myeloperoxidase activity and levels of tissue hydroxyproline were determined to assess neutrophil activation and collagen accumulation, respectively. Treatment with SPC markedly reduced serum transaminase activities as compared to BDL rats. Sphingosylphosphorylcholine also inhibited the increase in liver malondialdehyde; nitric oxide levels significantly and also attenuated the depletion of superoxide dismutase in the liver after BDL. Similarly, the increase in tissue myeloperoxidase activity and hydroxyproline owing to BDL was also attenuated by the SPC treatment. These data were supported by histopathologic findings. The a-smooth muscle actin-positive cells in the BDL were observed to be reduced with the SPC treatment. In conclusion, these findings suggested that SPC can attenuate hepatic damage in extrahepatic cholestasis by prevention of oxidative stress, and inflammatory process. All these findings suggest that SPC may be a promising new therapeutic agent for cholestatic liver injury. (C) 2009 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.jpedsurg.2008.09.016
dc.identifier.endpage710en_US
dc.identifier.issn0022-3468
dc.identifier.issn1531-5037
dc.identifier.issue4en_US
dc.identifier.pmid19361629en_US
dc.identifier.scopus2-s2.0-63649129338en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage702en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpedsurg.2008.09.016
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23934
dc.identifier.volume44en_US
dc.identifier.wosWOS:000265467100006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSPCen_US
dc.subjectExtrahepatic Cholestasisen_US
dc.subjectImpaired Bile Flowen_US
dc.subjectBile Duct Ligationen_US
dc.subjectOxidative Stressen_US
dc.subjectLipid Peroxidationen_US
dc.subjectNitric-Oxideen_US
dc.subjectHepatic-Fibrosisen_US
dc.subjectCancer-Cellsen_US
dc.subjectActivationen_US
dc.subjectInhibitionen_US
dc.subjectTissueen_US
dc.subjectProliferationen_US
dc.subjectMechanismsen_US
dc.subjectExpressionen_US
dc.subjectCirrhosisen_US
dc.titleEffects of sphingosylphosphorylcholine against cholestatic oxidative stress and liver damage in the common bile duct ligated ratsen_US
dc.typeArticleen_US

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