Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study

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dc.authoridGulmez, Dolunay/0000-0001-9021-0439
dc.authoridErgin, Çağrı/0000-0001-7783-8723
dc.authoridAGCA, HARUN/0000-0002-2651-2034
dc.authoridAksoy, Seçil/0000-0002-3760-9755
dc.authoridEkşi, Fahriye/0000-0003-2245-7979
dc.authoridArikan-Akdagli, Sevtap/0000-0001-9807-6764
dc.authoridSig, Ali Korhan/0000-0003-2907-257X
dc.authorwosidGulmez, Dolunay/K-7287-2012
dc.authorwosidErgin, Çağrı/L-3988-2017
dc.authorwosidGürcan, Şaban/D-2754-2017
dc.authorwosidAGCA, HARUN/ISU-9626-2023
dc.authorwosidAksoy, Seçil/AAH-8540-2021
dc.authorwosidEkşi, Fahriye/AGV-1106-2022
dc.authorwosidTopaç, Tuncay/GQA-6410-2022
dc.contributor.authorEner, Beyza
dc.contributor.authorErgin, Cagri
dc.contributor.authorGulmez, Dolunay
dc.contributor.authorAgca, Harun
dc.contributor.authorTikvesli, Melek
dc.contributor.authorAksoy, Secil Ak
dc.contributor.authorOtkun, Muserref
dc.date.accessioned2024-06-12T11:20:46Z
dc.date.available2024-06-12T11:20:46Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.en_US
dc.description.sponsorshipBursa Uludag University Scientific Research Projects Commission [QUAP[T]-2015-5]; Ener Private Health Service Companyen_US
dc.description.sponsorshipThis work was partly supported by Bursa Uludag University Scientific Research Projects Commission (QUAP[T]-2015-5) and Ener Private Health Service Company.en_US
dc.identifier.doi10.1093/jac/dkac125
dc.identifier.endpage1898en_US
dc.identifier.issn0305-7453
dc.identifier.issn1460-2091
dc.identifier.issue7en_US
dc.identifier.pmid35445259en_US
dc.identifier.scopus2-s2.0-85133214337en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1894en_US
dc.identifier.urihttps://doi.org/10.1093/jac/dkac125
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25773
dc.identifier.volume77en_US
dc.identifier.wosWOS:000784578100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofJournal Of Antimicrobial Chemotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keywords]en_US
dc.titleFrequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre studyen_US
dc.typeArticleen_US

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