Investigation of the effects of pesticides on 'Jonagold' apple (Malus x domestica) polyphenol oxidase enzyme activity

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dc.authoridarabaci, gulnur/0000-0002-1190-5695
dc.authoridPaluzar, Hatice/0000-0001-9232-8748;
dc.authorwosidarabaci, gulnur/HSF-5994-2023
dc.authorwosidPaluzar, Hatice/IZQ-0711-2023
dc.authorwosidÇesko, Cengiz/AIA-6870-2022
dc.contributor.authorCesko, Cengiz
dc.contributor.authorGashi, Sami
dc.contributor.authorArabaci, Gulnur
dc.contributor.authorPaluzar, Hatice
dc.contributor.authorDurmishi, Berat
dc.contributor.authorBruci, Era
dc.contributor.authorVllasaliu, Fjolla
dc.date.accessioned2024-06-12T10:52:27Z
dc.date.available2024-06-12T10:52:27Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, the effects of a number of commonly used pesticides on the activity of the polyphenol oxidase (PPO) enzyme from pesticide-free and pesticide-applied 'Jonagold' apples were comparatively evaluated. Substrates 4-methylcatechol, pyrocatechol, pyrogallol and L-tyrosine were used to determine the substrate specificity of the PPO enzyme obtained from apple. According to the results, on the contrary, PPO enzyme from 'Jonagold' apple did not show any activity against L-tyrosine substrate which is a monophenolic substrate, the enzyme had high affinity against 4-methylcatechol, pyrocatechol and pyrogallol which are di and tri phenolic substrates. Km values of PPO enzyme obtained from pesticide-free apples against 4-methylcatechol, pyrocatechol, and pyrogallol substrates were determined as followed 0.27 mM, 2.27 mM, and 0.37 mM, respectively. Vmax values were found as 0.133 mM/min, 0.081 mM/min, and 0.051 mM/min. Optimum pH values were found to be 4.5 for 4-methylcatechol, 7.0 for pyrocatechol, and 7.5 for pyrogallol. Optimum temperature values were determined as 40 degrees C for 4-methylcatechol, 10 degrees C for pyrocatechol, and 50 degrees C for pyrogallol. Km values for PPO enzyme activity obtained from pesticide-treated apples against 4-methylcatechol, pyrocatechol, and pyrogallol substrates were 0.98 mM, 3.94 mM, and 0.37 mM, Vmax values were 0.08 mM/min, 0.02 mM/min, and 0.034 mM/min. Optimum pH values were found to be 7.0 for 4-methylcatechol and pyrocatechol and 7.5 for pyrogallol. Optimum temperature values were determined as 50 degrees C for 4-methylcatechol, 30 degrees C for pyrocatechol, and 40 degrees C for pyrogallol. Overall, the results showed that the PPO enzyme from pesticide-free apples had higher activity than pesticide-treated apples. The effects of metals and storage stability on PPO enzyme activity were also investigated. The results reveal that pesticide use affects PPO enzyme activity. The obtained data bring to light new pesticide functional features of great interest in the medicinal, agro-chemical and environmental circles.en_US
dc.description.sponsorshipMinistry of Education, Science and Technology of Kosovoen_US
dc.description.sponsorshipWe would like to thank the Ministry of Education, Science and Technology of Kosovo for financially supporting our project in 2020.en_US
dc.identifier.doi10.55730/1300-011X.3064
dc.identifier.endpage66en_US
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85148686001en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage57en_US
dc.identifier.trdizinid1159628en_US
dc.identifier.urihttps://doi.org/10.55730/1300-011X.3064
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1159628
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18698
dc.identifier.volume47en_US
dc.identifier.wosWOS:000933796700006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Agriculture And Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAppleen_US
dc.subjectKinetic Characterizationen_US
dc.subjectPurificationen_US
dc.subjectInhibitionen_US
dc.subjectTyrosinase Biosensoren_US
dc.subjectInhibitionen_US
dc.subjectResiduesen_US
dc.titleInvestigation of the effects of pesticides on 'Jonagold' apple (Malus x domestica) polyphenol oxidase enzyme activityen_US
dc.typeArticleen_US

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