Utilization of bentonite as a support material for immobilization of Candida rugosa lipase

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dc.contributor.authorYesiloglu, Y
dc.date.accessioned2024-06-12T10:55:35Z
dc.date.available2024-06-12T10:55:35Z
dc.date.issued2005
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBentonite is an inexpensive, matrix for enzyme immobilization and has been frequently utilized for this purpose. In addition to its low cost, bentonite has several advantages for use as a support; including its lack of toxicity and chemical reactivity, allowing easy fixation of enzymes. In this article, Candida rugosa lipase was non-covalently immobilized on bentonite. The properties of immobilized enzyme were defined. Optimum pH and water content were determined as 7.5 and 100 mu L, respectively. The patterns of heat stability indicated that the immobilization process tends to stabilize the enzyme. Half-lives of the immobilized enzyme at 50, 60, and 70 degrees C were 45, 15, and 4 min, respectively, whereas for the soluble free lipase,it was 17 min at 50 degrees C. Kinetics were tested at 37 degrees C following the hydrolysis of olive oil and obeys the Lineweaver-Burk type of rate equation. The K-m was 4 x 10(-2) mM and the V-max was 15 mmol h(-1) mg(-1). (c) 2004 Elsevier Ltd. All r ights reserved.en_US
dc.identifier.doi10.1016/j.procbio.2004.08.008
dc.identifier.endpage2159en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-13844311537en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2155en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2004.08.008
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19481
dc.identifier.volume40en_US
dc.identifier.wosWOS:000227569200019en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCandida Rugosa Lipaseen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectBentoniteen_US
dc.subjectEnzymesen_US
dc.subjectOrganic Solventsen_US
dc.subjectKinetic Propertiesen_US
dc.subjectHydrolysisen_US
dc.subjectEnzymeen_US
dc.subjectBeadsen_US
dc.titleUtilization of bentonite as a support material for immobilization of Candida rugosa lipaseen_US
dc.typeArticleen_US

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