Some biochemical properties of lipase from bay laurel (Laurus nobilis L.) seeds

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorIsbilir, Sebnem Selen
dc.contributor.authorOzcan, Hakki M.
dc.contributor.authorYagar, Hulya
dc.date.accessioned2024-06-12T10:54:14Z
dc.date.available2024-06-12T10:54:14Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractLipase was isolated from bay laurel (Laurus nobilis L.) seeds, some biochemical properties were determined. The bay laurel oil was used as the substrate in all experiments. The pH optimum was found to be 8.0 in the presence of this substrate. The temperature optimum was 50 degrees C. The specific activity of the lipase was found to be 296 U mg protein(-1) in optimal conditions. The enzyme activity is quite stable in the range of pH 7.0-10. The enzyme was stable for 1 h at its optimum temperature, and retained about 68% of activity at 60 degrees C during this time. K-m and V-max values were determined as 0.975 g and 1.298 U mg protein(-1), respectively. Also, storage stability and metal effect on lipolytic activity were investigated. Enzyme activity was maintained for 9, 12, and 42 days at room temperature, 4 and -20 degrees C, respectively. Ca2+, Co2+, Cu2+, Fe2+, and Mg2+ lightly enhanced bay laurel lipase activity.en_US
dc.identifier.doi10.1007/s11746-007-1176-8
dc.identifier.endpage233en_US
dc.identifier.issn0003-021X
dc.identifier.issn1558-9331
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-39149144776en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage227en_US
dc.identifier.urihttps://doi.org/10.1007/s11746-007-1176-8
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18981
dc.identifier.volume85en_US
dc.identifier.wosWOS:000253398400005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of The American Oil Chemists Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBay Laurel (Laurus Nobilis L.)en_US
dc.subjectLipaseen_US
dc.subjectDefattingen_US
dc.subjectThermal Stabilityen_US
dc.subjectMetal Ionsen_US
dc.subjectExtractsen_US
dc.subjectLatexen_US
dc.subjectInteresterificationen_US
dc.subjectPurificationen_US
dc.titleSome biochemical properties of lipase from bay laurel (Laurus nobilis L.) seedsen_US
dc.typeArticleen_US

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