Investigation of Pathogenic Escherichia coli Strains in Patients with Diarrhea

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dc.contributor.authorTutak, Gulten Aydin
dc.contributor.authorTugrul, Hamdi Murat
dc.date.accessioned2024-06-12T10:59:31Z
dc.date.available2024-06-12T10:59:31Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe role of certain serogroups and serotypes of Escherichia coli in the etiology of gastroenteritis is increasingly appreciated. It is important to detect the virulence factors of diarrheagenic E.coli strains that differentiate them from nonpathogenic members of normal intestinal flora for the diagnosis and treatment. The aims of this study were to determine the serotypes of E.coli isolates that cause gastroenteritis and to investigate the presence of virulence genes by polymerase chain reaction (PCR). A total of 202 watery, bloody or mucoid stool samples sent to microbiology laboratory collected from patients with diarrhea who were admitted to outpatient clinics of Trakya University Health Research and Application Hospital between February to October 2009, were included in the study. A total of 254 predominantly grown E.coli strains have been isolated and identified with conventional methods from the cultures of those 202 samples. All strains were tested by slide agglutination (SA) that includes 6 units of 0 serogroups polyvalent antisera of enteropathogenic E.coli (EPEC), enterotoxigenic E.coli (ETEC) and enteroinvasive E.coli (EIEC). The samples which yielded positive results with SA test and the same number of negative samples selected with mapping method as controls were studied for the presence of virulence genes belonging EPEC, ETEC and EIEC by conventional PCR. In the study, 14.3% (29/202) of the samples were serogrouped with SA, of them 13 (6.4%) were identified as EPEC, 11(5.4%) as EIEC and five (2.4%) as ETEC. Only five isolates belonging to EPEC serogroup could be defined by monovalent antiserum and they were all in 01 serogroup. Out of 29 pathogenic E.coli serotyped, 3 (10.3%) of them harbored the virulence genes of diarrheagenic strains. One sample which was positive for eaeA gene of EPEC, did not harbor bfpA and stx genes and was defined as atypical EPEC. Out of other two samples, one was positive for estA gene of ETEC and the other one for ial gene of EIEC. One strain serotyped as EPEC detected to carry estA gene of ETEC with PCR. All of the 29 control isolates that give negative results with polyvalent antisera were also negative for the presence of virulence genes. In conclusion, since serotyping and conventional PCR methods did not reveal similar results for the identification of pathogenic E.coli, multicenter and large-scaled studies performed with standardized methods are needed.en_US
dc.identifier.doi10.5578/mb.8822
dc.identifier.endpage129en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid25706738en_US
dc.identifier.scopus2-s2.0-84924808150en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage124en_US
dc.identifier.urihttps://doi.org/10.5578/mb.8822
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20477
dc.identifier.volume49en_US
dc.identifier.wosWOS:000350946600013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiarrheaen_US
dc.subjectPathogenic Escherichia Colien_US
dc.subjectSerotypingen_US
dc.subjectVirulence Genesen_US
dc.subjectPCRen_US
dc.subjectReal-Time Pcren_US
dc.subjectChildrenen_US
dc.subjectAssayen_US
dc.titleInvestigation of Pathogenic Escherichia coli Strains in Patients with Diarrheaen_US
dc.typeArticleen_US

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