Caffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cells

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dc.contributor.authorÖzgün, Gülben Sayılan
dc.contributor.authorÖzgün, Eray
dc.contributor.authorTabakçıoğlu, Kıymet
dc.contributor.authorGökmen, Selma Süer
dc.contributor.authorEskiocak, Sevgi
dc.contributor.authorÇakır, Erol
dc.date.accessioned2021-11-20T10:10:04Z
dc.date.available2021-11-20T10:10:04Z
dc.date.issued2017
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Anabilim Dalıen_US
dc.description.abstractBackground: Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 are antioxidant and anti-atherosclerotic structural high-density lipoprotein proteins that are mainly synthesized by the liver. No study has ever been performed to specifically examine the effects of caffeine on paraoxonase enzymes and on liver apolipoprotein A-1 protein levels. Aims: To investigate the dose-dependent effects of caffeine on liver apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels. Study Design: In vitro experimental study. Methods: HepG2 cells were incubated with 0 (control), 10, 50 and 200 ?M of caffeine for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide assay. ApolipoproteinA-1, paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting. Results: We observed a significant increase on apolipoprotein A-1 and paraoxonase-1 protein levels in the cells incubated with 50 µM of caffeine and a significant increase on paraoxonase-1 protein level in the cells incubated with 200 µM of caffeine. Conclusion: Our study showed that caffeine does not change paraoxonase-3 protein level, but the higher doses used in our study do cause an increase in both apolipoprotein A-1 and paraoxonase-1 protein levels in liver cellsen_US
dc.identifier.doi10.4274/balkanmedj.2016.1217en_US
dc.identifier.endpage539en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue6en_US
dc.identifier.pmid29215336en_US
dc.identifier.scopus2-s2.0-85038238617en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage534en_US
dc.identifier.trdizinid268517en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpZNE5URTNOdz09
dc.identifier.urihttps://hdl.handle.net/20.500.14551/5491
dc.identifier.volume34en_US
dc.identifier.wosWOS:000423241000009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240608_ID_Qen_US
dc.subjectCerrahien_US
dc.titleCaffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cellsen_US
dc.typeArticleen_US

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