Intranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats

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dc.authoridOvali, Mehmet Akif/0000-0001-8740-6422
dc.authoridUZUN, Metehan/0000-0003-1406-5473
dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authorwosidaykora, damla/JRW-1473-2023
dc.authorwosidOvali, Mehmet Akif/AGT-5201-2022
dc.authorwosidUZUN, Metehan/JWR-0343-2024
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.contributor.authorMalcok, U. A.
dc.contributor.authorDoganlar, O.
dc.contributor.authorTufekcioglu, N. K.
dc.contributor.authorOvali, M. A.
dc.contributor.authorAykora, D.
dc.contributor.authorDoganlar, Z. B.
dc.contributor.authorBuyuk, B.
dc.date.accessioned2024-06-12T11:15:45Z
dc.date.available2024-06-12T11:15:45Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractEarly brain injury (EBI) in the first 24-72 h is the leading cause of mortality and disability related to subarachnoid hemorrhage (SAH). Both melatonin and microRNAs (miRs) are involved in the regulation of a number of neuronal molecular signaling procedures in the central nervous system, ranging from hypoxia, inflammation to neuronal apoptosis. The present study was performed to explore the effect of miRs-17/20 and combined treatment with melatonin on early brain injury after SAH and underlying molecular mechanisms in rats. In this study 54 Wistar albino rats were divided into six experimental groups: Sham, SAH, SAH + Melatonin, SAH+miRs-17/20 control, SAH+MEL+miRs-17/20, and SAH+MEL+miRs-17/20. The Garcia's Neurological Scoring Scale and motor coordination tests were used for clinical observation. H&E staining was performed to evaluate pathological score. The gene expression levels were determined by qRT-PCR and key proteins were quantitated by Western blot assay. miRs-17/20 with or without melatonin treatment suppressed the expression and activity of both the HIF1/VEGF/MMPs and the IL6R/JAK2/STAT3 axis. miRs-17/20 with or without melatonin treatment also mitigated the clinical impairment, pyknosis, and edema in the hippocampus and cortex and neurodegeneration induced by SAH. Our results show that miRs-17/20 alleviated EBI by reducing hypoxic conditions, hypoxia-induced molecular signaling, and neuronal apoptosis.en_US
dc.description.sponsorshipScientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey [TSA-2019-2943]en_US
dc.description.sponsorshipThis work was funded by the Scientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey (Project Number: TSA-2019-2943).en_US
dc.identifier.doi10.1007/s11094-023-02953-7
dc.identifier.endpage808en_US
dc.identifier.issn0091-150X
dc.identifier.issn1573-9031
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85174254620en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage793en_US
dc.identifier.urihttps://doi.org/10.1007/s11094-023-02953-7
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24060
dc.identifier.volume57en_US
dc.identifier.wosWOS:001084826300007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPharmaceutical Chemistry Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSubarachnoid Hemorrhage Early Brain Injuryen_US
dc.subjectMicrornas-17/20en_US
dc.subjectMelatoninen_US
dc.subjectMitochondrial Pathwayen_US
dc.subjectSignaling Pathwayen_US
dc.subjectMir-17-92 Clusteren_US
dc.subjectOxidative Stressen_US
dc.subjectMelatoninen_US
dc.subjectActivationen_US
dc.subjectApoptosisen_US
dc.subjectAutophagyen_US
dc.subjectModelen_US
dc.subjectHypoxia-Inducible-Factor-1-Alphaen_US
dc.titleIntranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Ratsen_US
dc.typeArticleen_US

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