Methylene blue increases contralateral testicular ischaemia-reperfusion injury after unilateral testicular torsion

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dc.authorwosidAydogdu, Nurettin/ABH-9224-2020
dc.contributor.authorInan, Mustafa
dc.contributor.authorBasaran, Umit N.
dc.contributor.authorDokmeci, Dikmen
dc.contributor.authorYalcin, Omer
dc.contributor.authorAydogdu, Nurettin
dc.contributor.authorTuran, Nesrin
dc.date.accessioned2024-06-12T10:58:24Z
dc.date.available2024-06-12T10:58:24Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractTesticular ischaemia-reperfusion injury is commonly seen in childhood. Infertility occurs in 25% of patients after unilateral testicular ischaemia. It is has been reported that methylene blue has a positive effect in the reparation of ischaemia-reperfusion injury in different tissues. Therefore, we hypothesized that methylene blue may prevent the hazardous effects of ischaemia-reperfusion injury in testicular tissue after unilateral testicular torsion. Thirty-two prepubertal Wistar-albino rats were divided into four groups. Testicular torsion was created by rotating the right testis 720 degrees in a clockwise direction for 5 h in all groups except for Group C, which was the sham control group. In Group T, bilateral orchiectomy was performed following the torsion period. In Group TD, both testes were removed 5 days after the torsion period. In Group MB, methylene blue (1 mg/kg, i.p.) was administered 40 min before detorsion and once daily over 5 days; then, both testes were harvested. Tissue levels of malondialdehyde (MDA), serum levels of creatine kinase (CK), mean testicular biopsy score (MTBS) and mean seminifer tubule diameter (MSTD) were determined. There was a significant difference in MTBS between Groups T and TD (P < 0.05) in both ipsilateral and contralateral testes. In the contralateral testis, treatment with methylene blue decreased MTBS and MSTD (P < 0.05) and increased MDA levels (P < 0.05). In Group T, mean serum CK concentrations were higher than in any of the other groups (P < 0.05). After 5 h of unilateral testicular torsion and a 5 day reperfusion period, serious tissue damage occurred on both the ipsilateral and contralateral sides. Serum CK concentrations may be an indicator for ischaemia, but not for ischaemia-reperfusion injury. Contrary to our hypothesis, methylene blue increased contralateral testicular damage after unilateral testicular torsion and exacerbated oxidative events.en_US
dc.identifier.doi10.1111/j.1440-1681.2007.04736.x
dc.identifier.endpage54en_US
dc.identifier.issn1440-1681
dc.identifier.issue1en_US
dc.identifier.pmid18047627en_US
dc.identifier.scopus2-s2.0-36649018702en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage50en_US
dc.identifier.urihttps://doi.org/10.1111/j.1440-1681.2007.04736.x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20059
dc.identifier.volume35en_US
dc.identifier.wosWOS:000251249600010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofClinical And Experimental Pharmacology And Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectContralateral Testisen_US
dc.subjectCreatine Kinaseen_US
dc.subjectIschaemia-Reperfusionen_US
dc.subjectJohnsen Scoreen_US
dc.subjectMalondialdehydeen_US
dc.subjectMethylene Blueen_US
dc.subjectTesticular Torsionen_US
dc.subjectSpermatic Cord Torsionen_US
dc.subjectPrepubertal Ratsen_US
dc.subjectNitric-Oxideen_US
dc.subjectBlood-Flowen_US
dc.subjectTestisen_US
dc.subjectAllopurinolen_US
dc.subjectAttenuationen_US
dc.subjectDamageen_US
dc.subjectModelen_US
dc.subjectDrugen_US
dc.titleMethylene blue increases contralateral testicular ischaemia-reperfusion injury after unilateral testicular torsionen_US
dc.typeArticleen_US

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