Karyotype and C-banding patterns of mitotic chromosomes in diploid bromegrass (Bromus riparius Rehm)

dc.contributor.authorTuna, M
dc.contributor.authorGill, KS
dc.contributor.authorVogel, KP
dc.date.accessioned2024-06-12T11:17:43Z
dc.date.available2024-06-12T11:17:43Z
dc.date.issued2001
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPrevious cytogenetic studies of the genus Bromus L. were limited to chromosome counts and construction of karyotypes on the basis of Feulgen staining. Since the chromosomes of Bromus are similar in morphology, these karyotypes are of limited use for chromosome identification and genome analysis. The objectives of this study were to develop and evaluate a Giemsa C-banding procedure to use in identification of individual bromegrass chromosomes and to develop a karyotype for diploid Bromus riparius Rehm. (2n = 14; PI 440215). All chromosomes had one or more C-bands which were located mainly at telomeric regions. A group (I) of four pairs of chromosomes had telomeric bands on only one arm and could be differentiated. In this group, one pair had an interstitial C-band along with a telomeric band, one pair had a nucleolus organizer region (NOR) at a subtelomeric location on the short arm, and the other two pair could be distinguished by centromere location. The other group (II) of three pairs or chromosomes had telomeric bands on both arms. The unequivocal identification of specific chromosomes of Group II was not possible in all cells because of their similarity and differential condensation of chromosomes. Chromosomes of both groups were either metacentric or submetacentric. The total length of individual chromosomes ranged from 5.58 to 6.87 mum and the arm ratios ranged from 1.02 to 1.5. The homologous chromosomes were paired and assigned numbers I to VII in decreasing length. A karyotype was constructed by means of the C-bands, mean chromosome lengths, and arm ratios. The C-banding procedure used in this study could be used to developed karyotypes for the other species of the genus Bromus and these C-banded karyotypes could be used to compare genomes within the genus.en_US
dc.identifier.doi10.2135/cropsci2001.413831x
dc.identifier.endpage834en_US
dc.identifier.issn0011-183X
dc.identifier.issn1435-0653
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0034949573en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage831en_US
dc.identifier.urihttps://doi.org/10.2135/cropsci2001.413831x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24797
dc.identifier.volume41en_US
dc.identifier.wosWOS:000170696200032en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofCrop Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBanded Karyotypesen_US
dc.subjectAlfalfaen_US
dc.subjectGenomeen_US
dc.titleKaryotype and C-banding patterns of mitotic chromosomes in diploid bromegrass (Bromus riparius Rehm)en_US
dc.typeArticleen_US

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