DNA content and ploidy determination of bromegrass germplasm accessions by flow cytometry

Basit Öğe Kaydı
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.contributor.authorTuna, M
dc.contributor.authorVogel, KP
dc.contributor.authorArumuganathan, K
dc.contributor.authorGill, KS
dc.date.accessioned2024-06-12T11:19:29Z
dc.date.available2024-06-12T11:19:29Z
dc.date.issued2001
dc.departmentTrakya Üniversitesien_US
dc.description.abstractSpecies of the genus Bromus represent ploidy states from diploid to decaploid. Ploidy determination of Bromus germplasm is necessary before it can be effectively used in breeding or genetic studies. The objective of this study was to characterize the ploidy of 322 accessions of four Bromus species [Bromus inermis Leyss, B. riparius Rehm, B. biebersteinii Roem and Schult., and B. inermis ssp. pumpellianus (Scribn) Wagnon] that are in the USDA National Plant Germplasm System (NPGS). Flow cytometry was used to determine DNA content of 10 plants of each accession. Mean DNA contents were correlated to ploidy level with root tip chromosome counts on selected accessions whose DNA content indicated that they represented different ploidy levels. On the basis of DNA content (pg 2C(-1) = DNA content of a diploid somatic nucleus) and chromosome counts, mean DNA content and chromosome number was 22.62 pg 2C(-1) for octaplold B. biebersteinii (2n = 8x = 56), 26.07 pg 2C(-1) for decaploid B. biebersteinii (2n = 10x 70), 11.74 pg 2C(-1) for tetraploid B. inermis (2n = 4x = 28), 22.28 pg 2C(-1) for octaploid B. inerm is (2n = 8x 56). 22.72 pg 2C(-1) for octaploid B. inermis ssp. pumpellianus (2n 8x = 56). 26.5 pg 2C(-1) for decaploid B. inermis ssp.pumpellianus (2n = 10x = 70), 6.14 pg 2C(-1) for diploid B. riparius (2n = 2x = 14), 22.15 pg 2C(-1) for octaploid B. riparius (2n = 8x = 56), and 26.64 pg 2C(-1) for decaploid B. riparius (2n = 10x = 70). Standard deviations of the mean values were 0.88 pg 2C(-1) or less. Most B. inermis and B. inermis ssp. pumpellianus accessions were octaploid (93.75%), while the majority of the B. riparius and B. biebersteinii were decaploid (92.30%). The B. inermis and related species in the USDA NPGS were collected primarily from areas in the former USSR. The NPGS bromegrass germplasm could be enhanced by collections from western and central Europe, the Middle East, and China.en_US
dc.identifier.doi10.2135/cropsci2001.4151629x
dc.identifier.endpage1634en_US
dc.identifier.issn0011-183X
dc.identifier.issn1435-0653
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-0034803371en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1629en_US
dc.identifier.urihttps://doi.org/10.2135/cropsci2001.4151629x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25222
dc.identifier.volume41en_US
dc.identifier.wosWOS:000176236800040en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofCrop Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNuclear-Dnaen_US
dc.subjectBromus-Inermisen_US
dc.subjectHybridsen_US
dc.titleDNA content and ploidy determination of bromegrass germplasm accessions by flow cytometryen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu