Evaluation of The Effect of Topotecan, Carvacrol, Epigallocatechin Gallate and Their Combination on Apoptotic Process in Neuroblastoma and Astrocyte Cells

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dc.contributor.authorOltulu, Çağatay
dc.contributor.authorAkıncı, Melek
dc.contributor.authorBakar, Elvan
dc.date.accessioned2024-06-12T10:05:40Z
dc.date.available2024-06-12T10:05:40Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractNeuroblastoma is a sympathetic nervous system cancer. Neuroblastoma most commonly affects children under five years of age and is the most common solid tumor in childhood. Topotecan is a topoisomerase 1 inhibitor. Carvacrol and epigallocatechin gallate are naturally derived substances with anticancer, antioxidant, and apoptotic properties. Our study aimed to evaluate the effects of topotecan, carvacrol, epigallocatechin gallate, topotecan+carvacrol, and topotecan+ epigallocatechin gallate combinations on the apoptotic signaling pathway. IC50 values were determined in neuroblastoma and healthy astrocyte cells using the MTT assay. Apoptotic mRNA expressions (topoisomerase 1 and 2, p53, BCL2, BAX, caspase 9, caspase 3, IL1, TNF?) in astrocytes and neuroblastoma cells at the neuroblastoma IC50 dose were analyzed using quantitative realtime PCR. Topotecan and carvacrol did not exhibit selective cytotoxic effects between healthy astrocytes and neuroblastoma cells. However, we found that the combination of topotecan+ epigallocatechin gallate and topotecan+carvacrol with epigallocatechin gallate showed selective cytotoxic effects on the neuroblastoma cell line compared to astrocyte cells. The obtained mRNA results can be interpreted as the initiation of apoptosis in neuroblastoma cells in the topotecan, carvacrol, epigallocatechin gallate, and topotecan+epigallocatechin gallate groups. Further studies are needed to investigate this matter in more detail.en_US
dc.identifier.doi10.55262/fabadeczacilik.1301973
dc.identifier.endpage384en_US
dc.identifier.issn1300-4182
dc.identifier.issn2651-4648
dc.identifier.issue3en_US
dc.identifier.startpage373en_US
dc.identifier.trdizinid1209398en_US]
dc.identifier.urihttps://doi.org/10.55262/fabadeczacilik.1301973
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1209398
dc.identifier.urihttps://hdl.handle.net/20.500.14551/13583
dc.identifier.volume48en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofFABAD Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleEvaluation of The Effect of Topotecan, Carvacrol, Epigallocatechin Gallate and Their Combination on Apoptotic Process in Neuroblastoma and Astrocyte Cellsen_US
dc.typeArticleen_US

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