Increased sensitivity of Trichomonas vaginalis isolation from vaginal secretions by subsequent blind passage of preliminary negative cultures
Küçük Resim Yok
Tarih
2005
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Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Trichomonas vaginalis is a vaginal protozoon causing the most prevalent nonviral sexually transmitted disease. Culture is considered as the most reliable and gold standard among conventional diagnostic methods in the detection of T. vaginalis. In the present study, the diagnostic effect of subsequent blind culturing of vaginal secretion samples in Diamond's complete medium at every 48 h was investigated in 93 women. Vaginal secretion samples were examined by wet mount and inoculated into Diamond's complete medium. All tubes were subcultured to a new culture tube blindly 5 times at 48-h interval and checked microscopically everyday for the presence of T. vaginalis trophozoites. Three of 93 women were found to be positive both by wet mount and standard culture methods. Five more positive results were detected with subsequent blind culturing among 90 negative results. This system appears to be a useful method for the detection of T. vaginalis. © 2005 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Culture; Diagnosis; Trichomonas Vaginalis, Article; Confidence Interval; Controlled Study; Culture Medium; Diagnostic Procedure; Female; Human; Inoculation; Major Clinical Study; Microorganism Detection; Nonhuman; Priority Journal; Reliability; Trichomonas Vaginalis; Trophozoite; Vagina Secretion; Adolescent; Adult; Animals; Culture Media; Female; Humans; Middle Aged; Parasitology; Pregnancy; Pregnancy Complications, Infectious; Sensitivity And Specificity; Serial Passage; Trichomonas Vaginalis; Trichomonas Vaginitis; Vagina
Kaynak
Diagnostic Microbiology and Infectious Disease
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
52
Sayı
1
