Genotoxic and Apoptotic Effects of Heavy Metal Mixture on Human Aortic Vascular Smooth Muscle Cell Line
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Tarih
2019
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Heavy metals are the most common pollutantswith specific gravity greater than 5g cm-3. Human exposureto heavy metals occurs mostly through the consumption offood and water containing heavy metals. Metals that enter thebody via drinking water may cause genotoxicity and variousdiseases. Therefore, permissible limits of heavy metals inwater for human consumption have been determined by lawsand regulations. Our study aimed to investigate the effectsof a mix of metals (Cu, Zn, Pb and Fe) at permissible limitsin drinking water on aortic smooth muscle cells (TG-HAVSMC)with at molecular level.Material-Method: A metal mix at the permissible concentrationfor drinking water and 10-fold higher concentration wereexposed to human aortic smooth muscle for 24 and 48 hours, andgene expression of antioxidant enzymes (CuZn-SOD, Mn-SOD,CAT, GST), heat-shock proteins (HSP27, HSP60, HSP70) andapoptosis pathway (p53, PUMA, BCL2, BAX, Cyt-C, Casp-3,TNF-?, Casp-8, DR4, DR5) cell cycle control (p21) and DNArepair (EXO1) were investigated in addition to RAPD-DNApolymorphism.Results: It was determined that heavy metal exposure causedincrease in the DNA polymorphism, apoptotic cell ratios andenhanced antioxidant and apoptosis pathway gene expressionin cells.Conclusions: In conclusion, the mix consisting of heavy metalsat permissible limits in drinking water has been shown to causeoxidative stress, genotoxicity and apoptosis in human aorticsmooth muscle cells. Considering that apoptosis in vascularsmooth muscle cells plays a role in the pathophysiology ofdiseases such as arteriosclerosis and hypertension, we believeit would be beneficial to assess these limits through extensiveinvestigations in further studies.
Açıklama
Anahtar Kelimeler
Kaynak
Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi
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Scopus Q Değeri
Cilt
10
Sayı
3
