Expression and regulation of c-Jun N-terminal kinase (JNK) in endometrial cells in vivo and in vitro

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dc.authoridAtabekoglu, Cem Somer/0000-0003-0264-0709
dc.authoridYen, Chih-Feng/0000-0002-5419-2605
dc.authoridYen, Chih-feng/0000-0002-5419-2605
dc.authorwosidAtabekoglu, Cem Somer/AAI-8767-2020
dc.authorwosidYen, Chih-Feng/AAD-4023-2021
dc.authorwosidYen, Chih-feng/GQI-2704-2022
dc.contributor.authorKizilay, Gulnur
dc.contributor.authorCakmak, Hakan
dc.contributor.authorYen, Chih-Feng
dc.contributor.authorAtabekoglu, Cem
dc.contributor.authorArici, Aydin
dc.contributor.authorKayisli, Umit Ali
dc.date.accessioned2024-06-12T11:08:08Z
dc.date.available2024-06-12T11:08:08Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractJNK(c-Jun N-terminal kinase) is one of the main types of mitogen-activated protein kinases. JNK modulates inflammation and apoptosis in response to stress. Our hypothesis is that temporal and spatial changes in JNK activity regulate inflammation in human endometrium and that fluctuation in estrogen and progesterone levels may play a role in JNK activation. Therefore, we aimed to determine total-(t-) and active-(phosphorylated, p-) JNK expression in endometrial tissues in vivo by immunohistochemistry, and in vitro by immunocytochemistry and Western blot analysis. Immunohistochemistry revealed moderate cytoplasmic and nuclear t-JNK immunoreactivity, and mostly nuclear p-JNK immunoreactivity throughout the menstrual cycle and early pregnancy. The highest p- and t-JNK immunoreactivity was detected in late secretory phase (P < 0.05). We observed that endometrial stromal cell (ESC)s showed a significant increase in p-JNK expression following 48 h of estrogen combined with progesterone (E-2 + P-4) withdrawal from the culture conditions, compared to control and non-withdrawal groups (P < 0.05). Upon treatment with JNK inhibitor SP600125, we observed a significantly decreased interleukin (IL)-8 level (P < 0.05) in the presence and absence of E-2. These results demonstrate that JNK expression increases during the late secretory phase when the inflammatory response is highest. Inhibition of IL-8 expression by SP600125 suggests that JNK is involved in regulation of proinflammatory mediators of endometrium.en_US
dc.identifier.doi10.1007/s00418-008-0421-z
dc.identifier.endpage771en_US
dc.identifier.issn0948-6143
dc.identifier.issn1432-119X
dc.identifier.issue4en_US
dc.identifier.pmid18506470en_US
dc.identifier.scopus2-s2.0-51249123980en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage761en_US
dc.identifier.urihttps://doi.org/10.1007/s00418-008-0421-z
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22300
dc.identifier.volume130en_US
dc.identifier.wosWOS:000258901000013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofHistochemistry And Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMAPKen_US
dc.subjectJNKen_US
dc.subjectIL-8en_US
dc.subjectMenstrual Cycleen_US
dc.subjectEndometriumen_US
dc.subjectActivated Protein-Kinaseen_US
dc.subjectFas Ligand Systemen_US
dc.subjectStromal Cellsen_US
dc.subjectMap Kinasesen_US
dc.subjectGene-Expressionen_US
dc.subjectInterleukin-8en_US
dc.subjectApoptosisen_US
dc.subjectPathologyen_US
dc.subjectEstrogenen_US
dc.subjectPathwayen_US
dc.titleExpression and regulation of c-Jun N-terminal kinase (JNK) in endometrial cells in vivo and in vitroen_US
dc.typeArticleen_US

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