Anti-inflammatory effect of hydrogen sulfide donor sodium-sulfide in an experimental mouse model of contact hypersensitivity

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dc.authoridGunduz, Ozgur/0000-0002-2470-3021
dc.authoridErcetin, Deniz/0000-0001-9713-044X
dc.authorwosidGunduz, Ozgur/A-2351-2016
dc.authorwosidGÜNDÜZ, Özgür/AAH-8717-2019
dc.contributor.authorOzlen, Nermin
dc.contributor.authorErcetin, Deniz
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorGunduz, Ozgur
dc.date.accessioned2024-06-12T10:51:13Z
dc.date.available2024-06-12T10:51:13Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Hydrogen sulfide (H2S), synthesized by most immune cells, has been shown to exert anti-inflammatory and antipruritic effects. The effect of H2S on allergic contact dermatitis (ACD), an inflammatory skin disease that negatively affects the quality of life, is unknown. Objectives: We planned to investigate the antipruritic and anti-inflammatory effects of the H2S donor sodium sulfide (Na2S) in the experimental mouse model of contact hypersensitivity (CHS), which is widely used for ACD research. Methods: CHS was created in Balb/c mice using 1-fluoro-2, 4-dinitrobenzene. Na2S was administered systemically (0.2-2-20 mg/kg/i.p.) and locally (1-3-10 nmol/both ear/i.d.) at 3 h and 25 h after the challenge. Ear thickness and the number of scratches were determined at 24 h and 48 h following the challenge. Ear tissue and serum interferon-gamma, interleukin (IL)-2, IL-4, and IL-5 cytokine levels were evaluated by enzyme-linked immunosorbent assay (ELISA). H and E staining was performed for histopathological studies. CD4(+) and CD8(+) T cells located in the skin were examined by immunohistochemical staining. Results: Locally (1-3-10 nmol/ear/i.d., P < 0.001, P < 0.0001, P < 0.0001, respectively) and systemically (2-20 mg/kg/i.p., P < 0.01, P < 0.0001, respectively), Na2S administration decreased ear thickness dose dependently. Local (1-3-10 nmol/ear/i.d.) Na2S treatment decreased serum IL-2 levels (P < 0.01, P < 0.05, and P < 0.01, respectively). Na2S administered locally (3-10 nmol/ear/i.d., P < 0.05) and systemically (20 mg/kg/i. p., P < 0.05) decreased the number of CD4(+) T lymphocytes. Conclusion: Locally and systemically administered Na2S reduces ear thickness, which is one of the symptoms of CHS, probably by preventing CD4(+) T lymphocyte infiltration and proliferation and decreasing IL-2 synthesis.en_US
dc.description.sponsorshipTrakya University Research Council [TUBAP-2021/94]en_US
dc.description.sponsorshipThis work was supported by Trakya University Research Council (TUBAP-2021/94).en_US
dc.identifier.doi10.4103/ds.DS-D-22-00173
dc.identifier.endpage+en_US
dc.identifier.issn1027-8117
dc.identifier.issn2223-330X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85166200036en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage94en_US
dc.identifier.urihttps://doi.org/10.4103/ds.DS-D-22-00173
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18284
dc.identifier.volume41en_US
dc.identifier.wosWOS:001026544600005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofDermatologica Sinicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllergic Contact Dermatitisen_US
dc.subjectContact Hypersensitivityen_US
dc.subjectHydrogen Sulfideen_US
dc.subjectInflammationen_US
dc.subjectPruritusen_US
dc.subjectGene Expressionsen_US
dc.subjectInflammationen_US
dc.subjectAngiogenesisen_US
dc.subjectDermatitisen_US
dc.subjectMiceen_US
dc.subjectSkinen_US
dc.subjectElicitationen_US
dc.subjectChemokinesen_US
dc.titleAnti-inflammatory effect of hydrogen sulfide donor sodium-sulfide in an experimental mouse model of contact hypersensitivityen_US
dc.typeArticleen_US

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