Effects of Cannabinoid Modulation on Hypothalamic Nesfatin-1 and Insulin Resistance

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dc.authoridKaya, Oktay/0000-0001-9639-8022
dc.authoridBayram, Sinasi/0000-0003-4785-4751
dc.authoridGunduz, Ozgur/0000-0002-2470-3021
dc.authorwosidGÜNDÜZ, Özgür/AAH-8717-2019
dc.authorwosidKaya, Oktay/JGL-9169-2023
dc.authorwosidBayram, Sinasi/AAK-7568-2021
dc.authorwosidGunduz, Ozgur/A-2351-2016
dc.contributor.authorKaya, Oktay
dc.contributor.authorYilmaz, Makbule Elif
dc.contributor.authorBayram, Sinasi
dc.contributor.authorGunduz, Ozgur
dc.contributor.authorKizilay, Gulnur
dc.contributor.authorOzturk, Levent
dc.date.accessioned2024-06-12T10:55:36Z
dc.date.available2024-06-12T10:55:36Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBoth nesfatin-1 and cannabinoid systems involved in the regulation of sleep, metabolism, and food intake. The relationship between cannabinoid system and nesfatin-1 levels remains to be elucidated. This study investigated nesfatin-1 and insulin resistance in 72-h rapid eye movement (REM) sleep-deprived mice under the effects of cannabinoid, and cannabinoid receptors CB1R and CB2R blocking. Sixty mice were exposed to 72-h sleep deprivation. Groups and drug administrations were as follows: Group 1 (control) received injection of vehicle. Group 2 received WIN 55,212,2. Group 3 received AM251 (CB1R antagonist) followed by WIN 55,212,2 injection. Group 4 received SR144528 (CB2R antagonist) followed by WIN 55,212,2 injection. Group 5 received only AM251. Group 6 received only SR144528. Blood samples were collected 1 h after drug administration and prepared for biochemical measurements. Glucose levels were measured by glucometer, whereas insulin and nesfatin-1 levels were measured by ELISA. Central nesfatin-1 was also assessed using immunohistochemistry. One-way analysis of variance together with post hoc Tukey's test was used for inter-group comparisons. Serum nesfatin-1 levels were comparable in all study groups. Brain nesfatin-1 immune-positive cell count was lower in WIN group compared to controls. The administration of CB1R or CB2R antagonist prevented reduction in nesfatin-1-positive cell count. Insulin resistance was higher in WINCB2 and CB2 groups than in control and WINCB1 groups. Cannabinoid treatment reduced nesfatin-1 immunoreactivity in the central nervous system and this effect was prevented by either CB1R or CB2R antagonist pretreatment. Insulin resistance might be related to CB2 receptor activation which was independent from central nesfatin-1 immunoreactivity.en_US
dc.description.sponsorshipScientific Research Fund of Trakya University [TUBAP 2015/230]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Fund of Trakya University (TUBAP 2015/230).en_US
dc.identifier.doi10.4103/CJP.CJP_50_19
dc.identifier.endpage187en_US
dc.identifier.issn0304-4920
dc.identifier.issn2666-0059
dc.identifier.issue5en_US
dc.identifier.pmid31670281en_US
dc.identifier.scopus2-s2.0-85074293712en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage182en_US
dc.identifier.urihttps://doi.org/10.4103/CJP.CJP_50_19
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19484
dc.identifier.volume62en_US
dc.identifier.wosWOS:000522851900002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofChinese Journal Of Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCannabinoid Receptor CB2en_US
dc.subjectCannabinoid Receptorsen_US
dc.subjectInsulin Resistanceen_US
dc.subjectNesfatin-1en_US
dc.subjectSleep Deprivationen_US
dc.subjectSleep-Deprivationen_US
dc.subjectGlucose-Homeostasisen_US
dc.subjectParadoxical Sleepen_US
dc.subjectSatiety Moleculeen_US
dc.subjectCb1 Receptoren_US
dc.subjectObesityen_US
dc.subjectSensitivityen_US
dc.subjectNeuronsen_US
dc.subjectStressen_US
dc.titleEffects of Cannabinoid Modulation on Hypothalamic Nesfatin-1 and Insulin Resistanceen_US
dc.typeArticleen_US

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