Meme kanseri oluşturulmuş farelerde N-asetilsistein in arginaz enzim aktivitesi, ornitin ve üre düzeyleri üzerine etkisi
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Dosyalar
Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Meme kanseri gün geçtikce ciddiyet kazanan bir sorun olarak karşımıza çıkmaktadır. Dünyada kadınlar arasında en sık görülen tümör olup, kadınlarda görülen kanser türlerinin yaklaşık %30'unu oluşturmaktadır. Arginaz enzimi üre döngüsünün son enzimidir. Nitrik oksit sentaz ile aynı substratı kullanarak L-argininden üre ve ornitin oluşumunu sağlamaktadır. Son yıllarda yapılan çalışmalarda kanser hastalarında serum ve doku arginaz enzim düzeyinin arttığı belirtilmekte ve arginazın kanser hastaları için belirleyici bir enzim olabileceği ileri sürülmektedir. L-sistein ve redükte glutatyon öncüsü olan N-asetilsistein'in antitoksik özelliği iyi bilinmektedir. Son yıllarda yapılan çalışmalarda da antimutajenik ve antikorsinojenik özellikleri sorgulanmaktadır. Bu çalışmada, meme kanseri geliştirilmiş dokulardaki arginaz enzim aktivitesi, ornitin ve üre düzeylerine, antikarsinojenik etkisi çeşitli çalışmalarda gösterilmiş olan N-asetilsitein tedavisinin etkileri araştırılmıştır. Ortalama ağırlığı 25-30 gram ağırlığında değişen, 8-10 haftalık, 20 tane erkek Balb/C 1 cinsi fare kullanılmıştır. Farelerin sol ayak iç bölgesine 0.2 ml Erhlich asit tümör hücresi enjekte edildi. 9. günde tümör çapı 1 cm olduğunda tümör ve tedavi grubu olmak üzere iki gruba ayrıldılar.15 gün boyunca tümör grubuna 0.2 ml. serum fizyolojik, tedavi grubuna ise 200 mg/kg N-asetilsistein intraperitoneal olarak verildi. Çalışmanın sonucunda; meme dokusu arginaz enzim aktivitesi (p<0.05), ornitin (p <0.01) ve üre (p<0.05) düzeyleri tedavi (tümör+N-asetilsistein) grubunda (n=9), tümör grubuna göre (n=10) anlamlı olarak düşük bulundu. Çeşitli kanser tiplerinde artan arginaz enzim aktivitesi, ornitin ve üre miktarlarının, N- asetilsistein tedavisi ile düşmesi, N-asetilsistein'in meme kanseri gelişiminin durdurulmasında koruyucu ve/veya tedavi edici bir ajan olarak kullanılabileceğini göstermektedir. N- asetilsistein'in bu etkilerinin ileri çalışmalarla irdelenmesi yararlı olacaktır.
Breast cancer is a problem which is becoming more serious day by day. It is the most frequent tumor found in women around the world and it forms almost 30 % of all the cancer types found in women. Arginase is the last enzyme of the urea cycle. It leads to the formation of urea and ornithine from L-arginine by using the same substrate with nitric oxide synthase. The recent studies have shown that serum and tissue arginase enzyme activity have increased in cancer patients. It was also reported in the same studies that arginase may be a significant enzyme for cancer patients. The antitoxic quality of N-acetylcysteine, which is the precursor of L-cysteine and reduced glutathione, is well-known. In the recent studies, the antimutagenic and anticarcinogenic effects of N- acetylcysteine have been widely investigated. The aim of this study was to investigate the possible effects of N-acetylcysteine which shown as an anticarcinogenic substance, on arginase enzyme activity, ornithine and urea levels in the experimental model of breast cancer in mice. 1 In the study, 20 male Balb/c mice were used. They were 8-10 months old and 25-30 grams on average. 0.2 ml Erhlich acid tumor cell was injected into the subcutan part of their left feet. On the 9th day, when the diameter of the tumor was 1 cm, the mice were grouped into two as tumor and treatment groups. For 15 days, 0.2 ml isotonic sodium chloride were applied to the mice in the tumor group and 200 mg/kg N-acetylcysteine were applied to the ones in the treatment group as intraperitoneal. The result of the study revealed that the breast tissue arginase enzyme activity (p<0.05), ornithine (p<0.01) and urea (p<0.05) levels in the treatment group (n=9) were significantly lower than the tumor group (n=10). It can be suggested that while decreasing the arginase enzyme activity, ornithine and urea levels which found to be increased in various cancer types, N-acetylcysteine may have a protective and/or therapeutic role on the breast cancer development. Those effects of N- acetylcysteine on cancer progress need more investigation.
Breast cancer is a problem which is becoming more serious day by day. It is the most frequent tumor found in women around the world and it forms almost 30 % of all the cancer types found in women. Arginase is the last enzyme of the urea cycle. It leads to the formation of urea and ornithine from L-arginine by using the same substrate with nitric oxide synthase. The recent studies have shown that serum and tissue arginase enzyme activity have increased in cancer patients. It was also reported in the same studies that arginase may be a significant enzyme for cancer patients. The antitoxic quality of N-acetylcysteine, which is the precursor of L-cysteine and reduced glutathione, is well-known. In the recent studies, the antimutagenic and anticarcinogenic effects of N- acetylcysteine have been widely investigated. The aim of this study was to investigate the possible effects of N-acetylcysteine which shown as an anticarcinogenic substance, on arginase enzyme activity, ornithine and urea levels in the experimental model of breast cancer in mice. 1 In the study, 20 male Balb/c mice were used. They were 8-10 months old and 25-30 grams on average. 0.2 ml Erhlich acid tumor cell was injected into the subcutan part of their left feet. On the 9th day, when the diameter of the tumor was 1 cm, the mice were grouped into two as tumor and treatment groups. For 15 days, 0.2 ml isotonic sodium chloride were applied to the mice in the tumor group and 200 mg/kg N-acetylcysteine were applied to the ones in the treatment group as intraperitoneal. The result of the study revealed that the breast tissue arginase enzyme activity (p<0.05), ornithine (p<0.01) and urea (p<0.05) levels in the treatment group (n=9) were significantly lower than the tumor group (n=10). It can be suggested that while decreasing the arginase enzyme activity, ornithine and urea levels which found to be increased in various cancer types, N-acetylcysteine may have a protective and/or therapeutic role on the breast cancer development. Those effects of N- acetylcysteine on cancer progress need more investigation.
Açıklama
Yüksek Lisans Tezi
Anahtar Kelimeler
Meme Kanseri, Arginaz, Ornitin, Üre, N-Asetilsistein, Breast Cancer, Arginase, Ornithine, Urea, N-Acetylcysteine
