RESPONSES OF ANTIOXIDANT ENZYMES AND HEAT SHOCK PROTEINS IN DROSOPHILA TO TREATMENT WITH A PESTICIDE MIXTURE

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.date.accessioned2024-06-12T11:12:19Z
dc.date.available2024-06-12T11:12:19Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe effects of a mixture of seven pesticides were examined on the expression of antioxidant enzymes, Mn superoxide dismutase (Mn-SOD), catalase (CAT), glutathione synthetase (GS), and heat shock proteins (HSP) 26, 60, 70 and 83 in adult fruit flies (Drosophila melanogaster Oregon R). The flies were reared under controlled conditions on artificial diets and treated with a mixture of seven pesticides (molinate, thiobencarb, linuron, phorate, primiphos-methyl, fenvalerate and lambda-cyhalothrin) commonly found in water, at concentrations of 0.1, 0.5 and 1 parts per billion (ppb) for 1 and 5 days. Quantitative real-time PCR (qRT-PCR) analysis of Mn-SOD, CAT and GS expression revealed that the analyzed markers responded significantly to pesticide-induced oxidative stress, in particular on the 5th day of treatment. On the 1st day of treatment, the relative expression of HSP26 and HSP60 genes increased only after exposure to the highest concentrations of pesticides, whereas HSP70 and HSP83 expression increased after exposure to 0.5 and 1 ppb. After five days of treatment, the expression of all HSP genes was increased after exposure to all pesticide concentrations. A positive correlation was determined between the relative expression levels of some HSPs (except HSP60), and antioxidant genes. The observed changes in antioxidant enzyme and HSP mRNA levels in D. melanogaster suggest that the permissible limits of pesticide concentrations for clean drinking water outlined in the regulations of several countries are potentially cytotoxic. The presented findings lend support for reevaluation of these limits.en_US
dc.description.sponsorshipT.R. State Planning Organization [2011K120390]en_US
dc.description.sponsorshipThe authors are grateful to Prof. Dr. Yener Yoruk (Rector of Trakya University, Edirne/Turkey) and the Technology Research and Application Center (TUTAGEM), which is funded by the T.R. State Planning Organization (Project Number: 2011K120390) for providing laboratory equipment.en_US
dc.identifier.doi10.2298/ABS141031046D
dc.identifier.endpage876en_US
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84950154980en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage869en_US
dc.identifier.urihttps://doi.org/10.2298/ABS141031046D
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23118
dc.identifier.volume67en_US
dc.identifier.wosWOS:000366794800012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovicen_US
dc.relation.ispartofArchives Of Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPesticideen_US
dc.subjectQrt-PCRen_US
dc.subjectAntioxidant Genesen_US
dc.subjectHSPen_US
dc.subjectDrosophilaen_US
dc.subjectGenotoxicity Assessmenten_US
dc.subjectDna-Damageen_US
dc.subjectStressen_US
dc.subjectMelanogasteren_US
dc.subjectExposureen_US
dc.subjectBenzeneen_US
dc.subjectTolueneen_US
dc.subjectHsp70en_US
dc.subjectModelen_US
dc.subjectNanoparticlesen_US
dc.titleRESPONSES OF ANTIOXIDANT ENZYMES AND HEAT SHOCK PROTEINS IN DROSOPHILA TO TREATMENT WITH A PESTICIDE MIXTUREen_US
dc.typeArticleen_US

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