THE PRODUCTION OF A NEW FUNGAL -AMYLASE DEGRADED THE RAW STARCH BY MEANS OF SOLID-STATE FERMENTATION
Küçük Resim Yok
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, it was intended to produce a new fungal amylase by solid-state fermentation and purification and also to determine some of its biochemical properties. It was found that Penicillium brevicompactum had the best enzyme activity according to screening methods with amylase degrading raw starch, and P. brevicompactum was selected as the amylase source. Wheat bran, rice husks, and sunflower oil meal were tested to determine the best solid substrate. Wheat bran was determined as the best of these. The fermentation conditions were optimized for the production of amylase. The optimum fermentation conditions were found to be an initial moisture level for the solid substrate of 55%, moistening agent of 0.1M sodium phosphate buffer (pH 5.0), incubation period of 7d, inoculum concentration of 2.5mL, and incubation temperature at 30 degrees C. Penicillium brevicompactum -amylase was purified 45.98 times by the starch affinity method. The Km and Vmax values of -amylase for soluble starch were 5.71mg/mL and 666.6U/mL, respectively. This amylase showed maximum activity at between 30 and 50 degrees C and at pH 5.0. Initial enzyme activity was kept at 100% after incubation at 30 degrees C for 45min. Enzyme was stable in the pH range of 4.0-5.0. This enzyme was activated by Mn2+, Cu2+, and Na+ ions, and was inhibited by Mg2+, K+, Fe3+, and ethylenediamine tetraacetic acid (EDTA). The molecular mass of P. brevicompactum -amylase was found to be 32.5kD by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis.
Açıklama
Anahtar Kelimeler
-Amylase, Penicillium Brevicompactum, Purification, Raw Starch Digesting, Solid-Substrate Fermentation, Thermostable Alpha-Amylase, Aspergillus-Oryzae, Purification, Bacillus, Hydrolysis, Enzyme
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
40
Sayı
3
