Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

Basit Öğe Kaydı
dc.authoridUz, Yesim/0000-0002-0381-4590
dc.authorwosidKilinc, Leyla K/I-8856-2017
dc.contributor.authorKilinc, Leyla
dc.contributor.authorUz, Yesim Hulya
dc.date.accessioned2024-06-12T10:58:58Z
dc.date.available2024-06-12T10:58:58Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: The present study aimed to investigate the possibility that curcumin (CMN) protects against methotrexate (MTX)-induced testicular damage by affecting the phospho-p38 (p-p38) mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) signaling pathways. Methods: Eighteen male Wistar albino rats were randomly divided into three groups. The control group was given an intragastric administration of dimethyl sulfoxide (DMSO) daily for 14 days, the MTX group was given a single intraperitoneal dose of MTX (20 mg/kg) on the 11th day, and the MTX+CMN group was given intragastric CMN (100 mg/kg/day, dissolved in DMSO) for 14 days and a single intraperitoneal dose of MTX (20 mg/kg) on the 11th day. At the end of the experiment, all animals were sacrificed and the testicular tissues were removed for morphometry, histology, and immunohistochemistry. Body and testicular weights were measured. Results: Body weights, seminiferous tubule diameter, and germinal epithelium height significantly decreased in the MTX group compared to the control group. Whereas, the number of histologically damaged seminiferous tubules and interstitial space width significantly increased in the MTX group. In addition, the number of p-p38 MAPK immunopositive cells and the immunoreactivity of NF-kappa B also increased in the MTX group compared to the control group. CMN improved loss of body weight, morphometric values, and histological damage due to MTX. CMN also reduced the number of p-p38 MAPK immunopositive cells and the NF-kappa B immunoreactivity. Conclusion: CMN may reduce MTX-induced testicular damage by suppressing the p38 MAPK and NF-kappa B signaling pathways.en_US
dc.description.sponsorshipTrakya University Scientific Research Projects [TUBAP 2013/126]en_US
dc.description.sponsorshipThis study was supported by Trakya University Scientific Research Projects (TUBAP 2013/126).en_US
dc.identifier.doi10.5653/cerm.2020.04105
dc.identifier.endpage220en_US
dc.identifier.issn2233-8233
dc.identifier.issn2233-8241
dc.identifier.issue3en_US
dc.identifier.pmid34352168en_US
dc.identifier.scopus2-s2.0-85114774833en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage211en_US
dc.identifier.urihttps://doi.org/10.5653/cerm.2020.04105
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20270
dc.identifier.volume48en_US
dc.identifier.wosWOS:000700862200005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Soc Reproductive Medicineen_US
dc.relation.ispartofClinical And Experimental Reproductive Medicine-Cermen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCurcuminen_US
dc.subjectMethotrexateen_US
dc.subjectNuclear Factor-Kappaen_US
dc.subjectP38en_US
dc.subjectTestisen_US
dc.subjectInduced Oxidative Stressen_US
dc.subjectGerm-Cellsen_US
dc.subjectApoptosisen_US
dc.subjectInjuryen_US
dc.subjectToxicityen_US
dc.subjectSpermatogenesisen_US
dc.subjectNanoparticlesen_US
dc.subjectModulationen_US
dc.subjectResistanceen_US
dc.subjectReversesen_US
dc.titleProtective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathwaysen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu