Alteration in cardiac PI3K/Akt/mTOR and ERK signaling pathways with the use of growth hormone and swimming, and the roles of miR21 and miR133

Basit Öğe Kaydı
dc.authoridDOĞAN, AYTEN/0000-0003-2525-785X
dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridPalabıyık, Orkide/0000-0002-3488-3740
dc.authoridTAYFUR, PINAR/0000-0002-2825-0760
dc.authoridvardar, selma arzu/0000-0002-1073-1718
dc.authorwosidDOĞAN, AYTEN/ABA-6754-2020
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidPalabıyık, Orkide/Q-2792-2019
dc.contributor.authorPalabiyik, Orkide
dc.contributor.authorTastekln, Ebru
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorTayfur, Pinar
dc.contributor.authorDogan, Ayten
dc.contributor.authorVardar, Selma Arzu
dc.date.accessioned2024-06-12T11:02:51Z
dc.date.available2024-06-12T11:02:51Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAthletes misuse recombinant human growth hormone (r-hGH) to enhance their performance. Although r-hGH is known to increase cardiac hypertrophy, the underlying molecular mechanism remains unclear. The aim of the present study was to investigate the role of r-hGH in cardiac intracellular signaling pathways and of miR-21 and miR-133 expression in rat hearts during exercise. A total of 36 adult male Sprague-Dawley rats were divided into sedentary control (SC, n=9), swimming exercise (SE, n=8), r-hGH (GH, n=10) and swimming exercise plus r-hGH (SE-GH, n=9) groups. The exercise groups completed a 1-h swimming exercise 5 times a week for 8 weeks. Subcutaneous r-hGH was administered as 0.3 mg/kg/day. Phosphoinositide-3-kinase (PI3K), serine/threonine protein kinase 1 (AKT1), extracellular signal-regulated kinase (ERK), microRNA (miR)-21 and miR-133 expression was evaluated in ventricular muscle by real-time quantitative polymerase chain reaction. Protein expression of PI3K, AKT1, ERK and mechanistic target of rapamycin (mTOR) was also assessed by immunohistochemistry. Statistical differences were analyzed by two-way ANOVA. PI3K and AKT1 expression and the gene and protein levels was notably increased in the SE-GH group compared with in SC ventricular tissues (P<0.05). mTOR protein expression was higher in the GH, SE and SE-GH groups compared with in the SC group (P<0.05, <0.05 and <0.001, respectively). ERK gene/protein expression was similar across all groups. miR-21 and miR-133 levels in ventricular muscle were higher in the SE and GH groups than those in the SC group. In summary, growth hormone application coupled with swimming exercise appeared to affect the PI3K/AKT/mTOR signaling pathway in the left ventricular tissue of rats; however, ERK signaling pathway appeared inactive in physiological left ventricular hypertrophy caused by swimming and GH administration over 8 weeks. Furthermore, GH treatment resulted in increased miR-21 and miR-133 expression. Future study by our group will aim to assess the effects of higher dose GH treatment.en_US
dc.description.sponsorshipTrakya University Scientific Research Projects Unit (Edirne, Turkey) [2015-36]en_US
dc.description.sponsorshipThe current study was funded by Trakya University Scientific Research Projects Unit (Project no. 2015-36; Edirne, Turkey).en_US
dc.identifier.doi10.3892/br.2018.1179
dc.identifier.endpage106en_US
dc.identifier.issn2049-9434
dc.identifier.issn2049-9442
dc.identifier.issue2en_US
dc.identifier.pmid30842884en_US
dc.identifier.scopus2-s2.0-85059674226en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage97en_US
dc.identifier.urihttps://doi.org/10.3892/br.2018.1179
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21443
dc.identifier.volume10en_US
dc.identifier.wosWOS:000460799200004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofBiomedical Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGrowth Hormoneen_US
dc.subjectMicrornaen_US
dc.subjectHearten_US
dc.subjectSwimmingen_US
dc.subjectCardiac Hypertrophyen_US
dc.subjectLeft-Ventricular Hypertrophyen_US
dc.subjectHeart-Failureen_US
dc.subjectMicrornasen_US
dc.subjectExerciseen_US
dc.subjectInhibitionen_US
dc.subjectActivationen_US
dc.subjectExpressionen_US
dc.subjectMechanismen_US
dc.subjectTargeten_US
dc.subjectMtoren_US
dc.titleAlteration in cardiac PI3K/Akt/mTOR and ERK signaling pathways with the use of growth hormone and swimming, and the roles of miR21 and miR133en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu