Production and characterization of lipase from Penicillium aurantiogriseum under solid-state fermentation using sunflower pulp

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dc.authoridPaluzar, Hatice/0000-0001-9232-8748
dc.authoridPALÜZAR, HATICE/0000-0001-9232-8748
dc.authoridTuncay, Didem/0000-0002-0936-0788
dc.authorwosidPaluzar, Hatice/IZQ-0711-2023
dc.authorwosidPALÜZAR, HATICE/AAO-9428-2021
dc.authorwosidTuncay, Didem/HZL-1046-2023
dc.contributor.authorPaluzar, Hatice
dc.contributor.authorTuncay, Didem
dc.contributor.authorAydogdu, Halide
dc.date.accessioned2024-06-12T11:07:40Z
dc.date.available2024-06-12T11:07:40Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractSolid-State Fermentation (SSF) defined as a system in which the growth of microorganisms occurs on a moist solid substrate, shows tremendous potential in applications of microbial enzyme production as a low-cost, low-energy option. In this work, the production of lipase from Penicillium aurantiogriseum by using SSF was investigated. For this purpose, the sunflower pulp, an agro-industrial by-product, was utilized as a solid substrate in the SSF method. The optimization and characterization studies for lipase extract were performed by using the p-nitrophenyl palmitate (pNPP) (C16) as substrate. The lipase activity was found as 29.6 +/- 0.11 U/mg protein at optimum conditions (50 mM of Tris-HCl buffer, pH 7.0, 40 degrees C). In thermal stability assay, the catalytic activities of lipase which are kept at 40, 50 and, 60 degrees C in a water bath for 1 h, were calculated as 100%, 60.48 +/- 1.3%, 32.67 +/- 0.2%, respectively. Also, the enzyme lost rapidly its activity within 15 min at 70 degrees C. The kinetic data, K (m) and V (max) , were determined as 0.17 mM and 2.9 mu M/min, respectively. Also, 10 mM of Ba+2 ion had a slight activating effect (15%) showed on lipase. Lipase kept about 50% of its activity at 4 degrees C at the end of the 30 days. The hydrolytic activities of lipase for commercial olive oil and sunflower oil were found as 3.52 +/- 0.1 U/mg and 3.90 +/- 0.12 U/mg, respectively. The SSF method had obvious potential for the sustainable and cost-effective production of lipase from P. aurantiogriseum. Also, these results confirmed that the sunflower pulp could be used as an alternative substrate to produce enzymes in SSF conditions.en_US
dc.identifier.doi10.1080/10242422.2021.1901888
dc.identifier.endpage342en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85103039197en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage333en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2021.1901888
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22140
dc.identifier.volume39en_US
dc.identifier.wosWOS:000632320000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis And Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLipaseen_US
dc.subjectSolid State Fermentationen_US
dc.subjectSunflower Pulpen_US
dc.subjectAgro Industrial By-Producten_US
dc.subjectLipase Characterizationen_US
dc.subjectPenicillium Aurantiogriseumen_US
dc.subjectExtracellular Lipaseen_US
dc.subjectCrude Lipaseen_US
dc.subjectPurificationen_US
dc.subjectRestrictumen_US
dc.subjectBabassuen_US
dc.subjectCakeen_US
dc.titleProduction and characterization of lipase from Penicillium aurantiogriseum under solid-state fermentation using sunflower pulpen_US
dc.typeArticleen_US

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