GUM ARABIC APPLICATION IMPROVE ANTHER CULTURE EFFICIENCY IN RICE

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dc.authorwosidSEN, AYSE/AAD-1878-2020
dc.contributor.authorSen, Ayse
dc.contributor.authorBeser, Necmi
dc.date.accessioned2024-06-12T10:51:53Z
dc.date.available2024-06-12T10:51:53Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe present study; seven rice genotypes (Osmancdc-97, Edirne, TG1, TG2, Sur C1, Luna C1 and Reiziq) and positive check Taipei 309 cultivars were used to investigate anther culture efficiency under different in vitro culture environments. For optimization step of calli induction from rice anthers, the spikes were exposed to 10 degrees C cold at 8 days. Afterwards, rice anthers were inoculated on 20 different media compositions. Three Turkish genotypes (Osmancik-97, TG1 and TG2) and Taipei 309 gave positive results in four of twenty culture compositions. The effect of putrescine, gum Arabic and CuSO4 additives and carbon sources, sucrose and maltose, in the calli induction culture media were observed as a genotyped-dependent. N-6 media consist of maltose, 2,4-dichlorophenoxyacetic acid (2,4-D) and gum Arabic showed maximum calli induction rate. Obtained-calli from these four cultivars were put on 10 different solidify Murashige and Skoog (MS) media compositions to obtain plantlets. Two Turkish genotypes (Osmancik-97 and TG1) and Taipei 309 gave positive results in one of ten culture compositions. MS basal salt mixture with 1-naphthaleneacetic acid (NAA), Kinetin, sucrose, myo-inositol and agar gave better results than others. During the optimization phase, irradiation of the rice spikes with low-dose gamma radiation did not give the expected result. In the study to determine the culture ability after optimization, Taipei 309 was more efficient than Osmancik-97 and TG1. It was determined that 23% of the obtained plantlets after flow cytometer analysis were haploid. About 82% of the plantlets transferred to the soil were set to seed. Additionally, gum-Arabic increased anther culture efficiency in rice.en_US
dc.description.sponsorshipIstanbul University [FDP-2017-21499, 20910]en_US
dc.description.sponsorshipThis study was supported by IAEA Research Contract No: 20910 and Research fund of Istanbul University by Project Number: FDP-2017-21499. For the analysis of the obtained paddy plantlets, a PARTEC branded Flow cytometry device in Tekirdag Namik Kemal University, Field Crops Department, Plant Genetics and Cytogenetics Laboratory was used. We thank to laboratory manager Prof. Dr. Metin TUNA for assisting us with this analysis.en_US
dc.identifier.endpage9877en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue9en_US
dc.identifier.startpage9867en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18509
dc.identifier.volume31en_US
dc.identifier.wosWOS:000862761500061en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnther Cultureen_US
dc.subjectCallus Inductionen_US
dc.subjectMedia Componentsen_US
dc.subjectPlant Regenerationen_US
dc.subjectRiceen_US
dc.subjectPlant-Regenerationen_US
dc.subjectEmbryogenesisen_US
dc.titleGUM ARABIC APPLICATION IMPROVE ANTHER CULTURE EFFICIENCY IN RICEen_US
dc.typeArticleen_US

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