Non-covalent immobilization of quince (Cydonia oblonga) polyphenol oxidase on alumina

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dc.contributor.authorYagar, H
dc.contributor.authorSagiroglu, A
dc.date.accessioned2024-06-12T10:51:20Z
dc.date.available2024-06-12T10:51:20Z
dc.date.issued2002
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPolyphenol oxidase enzyme purified partially from quince (Cydonia oblonga) was immobilized on alumina (Al2O3) by simple adsorption at pH 6.8. The properties of immobilized enzyme were compared to those of the free enzyme. Optimum pH (8.5) and temperature (45 degreesC) were determined, showing the alteration of pH and temperature profiles by immobilization. Catechol, L-DOPA, p-cresole and pyrogallol were tested as substrate and it was established that affinity was highest for catechol. K-m constant was 5 mM for catechol. Thermal and storage stability were carried out. It was observed that the immobilized enzyme had storage stability for a period of one year.en_US
dc.identifier.endpage+en_US
dc.identifier.issn1318-0207
dc.identifier.issn1580-3155
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0036971466en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage893en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18326
dc.identifier.volume49en_US
dc.identifier.wosWOS:000179843100016en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSlovensko Kemijsko Drustvoen_US
dc.relation.ispartofActa Chimica Slovenicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAluminaen_US
dc.subjectPolyphenol Oxidaseen_US
dc.subjectPurificationen_US
dc.subjectNon-Covalenten_US
dc.subjectImmbolizationen_US
dc.subjectTyrosinaseen_US
dc.subjectPhenolsen_US
dc.subjectPurificationen_US
dc.subjectElectrodesen_US
dc.subjectWateren_US
dc.titleNon-covalent immobilization of quince (Cydonia oblonga) polyphenol oxidase on aluminaen_US
dc.typeArticleen_US

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