Effects of ozone and photo-activated disinfection against Enterococcus faecalis biofilms in vitro
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Wolters Kluwer Medknow Publications
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Objectives: The purpose of this study was to compare the antibacterial effects of gaseous ozone (O-3) and photo-activated disinfection (PAD) methods against Enterococcus faecalis (E. faecalis) biofilms. Materials and Methods: Sixty-five human mandibular premolars with straight root canals were selected. After root canal preparation, the samples were sterilized and placed into eppendorf tubes with 1 mL brain heart infusion broth containing 1.5 x 10(8) colony-forming units (CFUs)/mL of E. faecalis. The contaminated samples were then divided into four groups (n = 15) according to the disinfection method used: Group 1, Saline (positive control); Group 2, NaOCl (negative control); Group 3, Gaseous O-3; and Group 4, PAD. Three non-contaminated teeth were used to control the infection and sterilization process. The CFUs were counted and the data were analyzed statistically. Results: There was a statistically significant difference between the experimental and control groups (P < 0.05). The saline group had the highest number of remaining microorganisms. Complete sterilization was achieved in the 2.5% NaOCl group. There were no statistically differences between PAD and gaseous O-3 (P > 0.05). Conclusion: Both PAD and gaseous O-3 have a significant antibacterial effect on infected root canals. However, 2.5% NaOCl was superior in terms of its antimicrobial abilities compared with the other disinfection procedures.
Description
Keywords
Biofilm, Ozone, Photo-Activated Disinfection, Root Canal Disinfection, Sodium-Hypochlorite, Antimicrobial Efficacy, Photodynamic Therapy, Ozonated Water, Root, Light, Chlorhexidine, Dentistry, Bacteria, Plasma
Journal or Series
Nigerian Journal Of Clinical Practice
WoS Q Value
Q4
Scopus Q Value
Q3
Volume
18
Issue
6
