Effects of methylene blue in reducing cholestatic oxidative stress and hepatic damage after bile-duct ligation in rats

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dc.authoridUmit, Hasan/0000-0002-3651-4180
dc.authoridUzun, Hafize/0000-0002-1347-8498
dc.authorwosidUmit, Hasan/T-6236-2019
dc.authorwosidAktas, Cevat/D-8468-2011
dc.authorwosidGuzel, Ahmet/AGE-2880-2022
dc.authorwosidUzun, Hafize/D-4811-2019
dc.contributor.authorAksu, Burhan
dc.contributor.authorUmit, Hasan
dc.contributor.authorKanter, Mehmet
dc.contributor.authorGuzel, Ahmet
dc.contributor.authorAktas, Cevat
dc.contributor.authorCivelek, Sabiha
dc.contributor.authorUzun, Hafize
dc.date.accessioned2024-06-12T11:08:26Z
dc.date.available2024-06-12T11:08:26Z
dc.date.issued2010
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the effects of methylene blue against cholestatic oxidative stress and liver damage after ligation of the common bile duct in male Wistar rats. Eight animals were included in each of the following five groups: untreated control, methylene blue control, sham-operated, bile-duct ligation, and bile-duct ligation plus methylene blue. Methylene blue was administered intraperitoneally for 14 days at a daily dose of 2 mg/kg per day. All rats were sacrificed 2 weeks following the experimental treatment and the livers of all groups were examined biochemically and histopathologically. The severity of cholestasis and hepatic injury were determined by changes in the plasma, including enzymatic activities: aspartate aminotransferase, alanine aminotransferase, gamma glutamine transferase, and also bilirubin levels. Malondialdehyde, nitric oxide and superoxide dismutase were measured to indicate the oxidative status in the liver tissue. Myeloperoxidase activity and levels of tissue hydroxyproline were determined as measures of neutrophil activation and collagen accumulation, respectively. Liver damage was significantly prevented in the bile-duct ligated rats treated with methylene blue compared with the control bile-duct ligated rats without methylene blue. Treatment with methylene blue markedly reduced activities of serum transaminase, gamma glutamine transferase and bilirubin levels as compared to bile-duct ligated rats without methylene blue. Positive immunolabelling for alpha-smooth muscle actin (alpha-SMA) was increased, especially in vascular smooth muscle cells, fibrotic septa and also around the proliferated bile ducts, after bile-duct ligation. Only weak alpha-SMA immunolabelling was seen in livers of rats treated with methylene blue. These results indicate that methylene blue can attenuate hepatic damage in extrahepatic cholestasis by reducing oxidative stress and inflammatory processes. (C) 2008 Elsevier GmbH. All rights reserved.en_US
dc.identifier.doi10.1016/j.acthis.2008.12.002
dc.identifier.endpage269en_US
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.issue3en_US
dc.identifier.pmid19217652en_US
dc.identifier.scopus2-s2.0-77950341021en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage259en_US
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2008.12.002
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22438
dc.identifier.volume112en_US
dc.identifier.wosWOS:000277578900007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbh, Urban & Fischer Verlagen_US
dc.relation.ispartofActa Histochemicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMethylene Blueen_US
dc.subjectExtrahepatic Cholestasisen_US
dc.subjectOxidative Stressen_US
dc.subjectHepatic Fibrosisen_US
dc.subjectBile-Duct Ligationen_US
dc.subjectAlpha-Smooth Muscle Actinen_US
dc.subjectRatsen_US
dc.subjectNitric-Oxideen_US
dc.subjectLiver-Injuryen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectBiliary Obstructionen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectFibrosisen_US
dc.subjectInhibitionen_US
dc.subjectCirrhosisen_US
dc.subjectFibroblastsen_US
dc.subjectAciden_US
dc.titleEffects of methylene blue in reducing cholestatic oxidative stress and hepatic damage after bile-duct ligation in ratsen_US
dc.typeArticleen_US

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