A new biosensor for osteoporosis detection

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorKabala, Sevilay Inal
dc.contributor.authorYagar, Hulya
dc.contributor.authorOzcan, Hakki Mevlut
dc.date.accessioned2024-06-12T11:11:46Z
dc.date.available2024-06-12T11:11:46Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractOsteoporosis is a disease that is characterized by deterioration of bone tissue and increased risk of fracture as it leads to a decrease in bone mineral density, which is an important public health problem. Today, bone mineral density is measured by radiological techniques. Alternative techniques are needed because of the disadvantages such as excessive radiation intake, the cost of radiological techniques, and the necessity for specialist personnel for the devices. The quantitative determination of biochemical markers that play a role in bone mineralization may be a good alternative for the osteoporosis diagnosis and especially in the follow-up of treatment. In this study, a specific and sensitive immunological biosensor, which quantitatively determines the osteocalcin molecule, has been developed to be used in the early osteoporosis diagnosis and to evaluate the response to the drug treatment. Anti-osteocalcin antibody was immobilized onto gold electrode surface via covalent immobilization method by using 6-mercaptohexanol, 1,4-butanedioldiglycidyl ether, ethanolamine, and glutaraldehyde. Immobilization steps and biosensor characterization were specified by cyclic voltammetry and electrochemical impedance spectroscopy. The detection time and range of Ocn biosensor were determined as 45 min and 10-60 pg mu L-1 Ocn concentration, respectively. The Ocn biosensor was successfully applied in artificial serum samples spiked with Ocn.en_US
dc.description.sponsorshipTrakya University Scientific Research Foundation [TUBAP-2016/106]en_US
dc.description.sponsorshipThis study is supported by Trakya University Scientific Research Foundation (Project Number: TUBAP-2016/106).en_US
dc.identifier.doi10.1080/10826068.2019.1587628
dc.identifier.endpage520en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue5en_US
dc.identifier.pmid30888246en_US
dc.identifier.scopus2-s2.0-85063079231en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage511en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2019.1587628
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22917
dc.identifier.volume49en_US
dc.identifier.wosWOS:000463296500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOsteocalcinen_US
dc.subjectOsteoporosisen_US
dc.subjectBiosensoren_US
dc.subjectElectrochemical Impedance Spectroscopyen_US
dc.subject1,4-Butanedioldiglycidyl Etheren_US
dc.subjectSelf-Assembled Monolayeren_US
dc.subjectPostmenopausal Osteoporosisen_US
dc.subjectBiochemical Markersen_US
dc.subjectEnzymatic Biosensoren_US
dc.subjectGold Electrodesen_US
dc.subjectBone Turnoveren_US
dc.subjectOsteocalcinen_US
dc.subjectSerumen_US
dc.subjectOsteopontinen_US
dc.subjectProteinen_US
dc.subjectCarbonen_US
dc.titleA new biosensor for osteoporosis detectionen_US
dc.typeArticleen_US

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