Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells

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dc.contributor.authorOzgun, Eray
dc.contributor.authorOzgun, Gulben Sayilan
dc.contributor.authorTabakcioglu, Kiymet
dc.contributor.authorGokmen, Selma Suer
dc.contributor.authorSut, Necdet
dc.contributor.authorEskiocak, Sevgi
dc.date.accessioned2024-06-12T11:07:41Z
dc.date.available2024-06-12T11:07:41Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractParaoxonase-1 (PON1) and paraoxonase-3 (PON3) are anti-atherosclerotic enzymes, synthesized primarily in liver and bound to HDL in circulation. The aim of the present study was to investigate the effects of therapeutic doses of lipoic acid on PON1 and PON3 protein levels, mRNA expression and arylesterase activity in liver. We treated HepG2 cells with 10, 40 and 200 mu M lipoic acid for 72 h. Cell viability was evaluated by 3-(4,5-dimethy1-2-thiazoly1)-2,5-dipheny1-2Htetrazolium bromide assay. PON1 and PON3 protein levels were measured by Western blotting, their mRNA expression was measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. 200 mu M lipoic acid caused a significant increase on PON1 and PON3 protein levels and arylesterase activity as compared with control, 10 mu M and 40 mu M lipoic acid treated cells. 200 mu M lipoic acid also caused a significant decrease on PON1 mRNA expression whereas on a significant increase PON3 mRNA expression as compared with control, 10 mu M and 40 mu M lipoic acid-treated cells. Our study showed that although lip oic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses.en_US
dc.description.sponsorshipScientific Research Appropriation of Trakya University, Turkey [TUBAP 2015/159]en_US
dc.description.sponsorshipThis study was supported by Scientific Research Appropriation of Trakya University (TUBAP 2015/159), Turkey. We would like to thank TUTAGEM laboratories.en_US
dc.identifier.doi10.4149/gpb_2017005
dc.identifier.endpage470en_US
dc.identifier.issn0231-5882
dc.identifier.issn1338-4325
dc.identifier.issue4en_US
dc.identifier.pmid28653653en_US
dc.identifier.scopus2-s2.0-85029894881en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage465en_US
dc.identifier.urihttps://doi.org/10.4149/gpb_2017005
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22143
dc.identifier.volume36en_US
dc.identifier.wosWOS:000412155000012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGeneral Physiol And Biophysicsen_US
dc.relation.ispartofGeneral Physiology And Biophysicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLipoic Aciden_US
dc.subjectParaoxonase-1en_US
dc.subjectParaoxonase-3en_US
dc.subjectArylesteraseen_US
dc.subjectLiveren_US
dc.subjectLow-Density-Lipoproteinen_US
dc.subjectBiological-Activityen_US
dc.subjectOxidative Stressen_US
dc.subjectGene-Expressionen_US
dc.subjectAtherosclerosisen_US
dc.subjectAntioxidanten_US
dc.subjectRatsen_US
dc.subjectFamilyen_US
dc.subjectEnzymeen_US
dc.subjectHdlen_US
dc.titleEffect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cellsen_US
dc.typeArticleen_US

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