Induction of symmetrical nucleus division and multicellular structures from the isolated microspores of lycopersicon esculentum mill

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dc.authorscopusid23097104100
dc.authorscopusid6602972194
dc.contributor.authorBal U.
dc.contributor.authorAbak K.
dc.date.accessioned2024-06-12T10:25:14Z
dc.date.available2024-06-12T10:25:14Z
dc.date.issued2005
dc.description.abstractEffects of lactalbumin hydolysate (LH), casein hydrolysate (CH) and a starvation pretreatment using mannitol in the induction of symmetrical nucleus division of uninucleate microspores and formation of multicellular structures from the isolated microspores of tomato were studied. Flower buds containing uninucleate microspores were incubated on media for the pretreatment containing either mannitol or sucrose at 10 °C for seven days. Subsequently microspores were isolated and cultured on NLN basic media in the presence of BAP, NAA, LH and CH. Microspores from the cv. Fantastic, cultured in the presence of BAP at 0.5 mg l?1 and NAA at 0.5, 1.0 and 1.5 mg l?1 together with LH at 2 or 4 ml l?1 proceeded in the sporophytical pathway, microspore nuclei dividing symmetrically. Formation of multicellular structures were induced which was also the case for other cultivars cultured in the NLN medium containing 0.5 mg l?1 BAP and 0.5 mg l?1 NAA together with CH either at 5 or 10 g l?1. Mannitol pretreatment induced microspore starvation and stress therefore microspores from the mannitol pretreatment were more responsive to the symmetrical division than those from the sucrose pretreatment. © 2005 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/13102818.2005.10817151
dc.identifier.endpage42en_US
dc.identifier.issn1310-2818
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-13844276138en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage35en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2005.10817151
dc.identifier.urihttps://hdl.handle.net/20.500.14551/16257
dc.identifier.volume19en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCasein Hydrolysate; Lactalbumin; Mannitol; Article; Cell Culture; Cell Division; Cell Isolation; Cell Nucleus; Cell Structure; Controlled Study; Cultivar; Flower; Microspore; Nonhuman; Plant Cell; Tomato; Lycopersicon Esculentumen_US
dc.titleInduction of symmetrical nucleus division and multicellular structures from the isolated microspores of lycopersicon esculentum millen_US
dc.typeArticleen_US

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