Production of Ricinoleic Acid from Castor Oil by Immobilised Lipases

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorSagiroglu, Ayten
dc.date.accessioned2024-06-12T10:55:56Z
dc.date.available2024-06-12T10:55:56Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPorcine pancreatic lipase (PPL), Candida rugosa lipase (CRL), and Castor bean lipase (CBL) were immobilized on celite by deposition from aqueous solution by the addition of hexane. Lipolytic performance of free and immobilized lipases were compared and optimizations of lipolytic enzymatic reactions conditions were performed by free and immobilized derivatives using olive oil as substrate. Afterwards, the influence on lipolysis of castor oil of free lipases and immobilized lipase derivatives have been studied in the case of production of ricinoleic acid. All of the lipases performances were compared and enzyme derivative was selected to be very effective on the production of ricinoleic acid by lipolysis reaction. Various reaction parameters affecting the production of ricinoleic acid were investigated with selected the enzyme derivative. The maximum ricinoleic acid yield was observed at pH 7-8, 50C, for 3hours of reaction period with immobilized 1,3-specific PPL on celite. The kinetic constants Km and Vmax were calculated as 1.6 10-4mM and 22.2mM from a Lineweaver-Burk plot with the same enzyme derivative. To investigate the operational stability of the lipase, the three step lipolysis process was repeated by transferring the immobilized lipase to a substrate mixture. As a result, the percentange of conversion after usage decreased markedly.en_US
dc.identifier.doi10.1080/10826060902800841
dc.identifier.endpage182en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue2en_US
dc.identifier.pmid19291579en_US
dc.identifier.scopus2-s2.0-65749083184en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage170en_US
dc.identifier.urihttps://doi.org/10.1080/10826060902800841
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19592
dc.identifier.volume39en_US
dc.identifier.wosWOS:000264224600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCastor Oilen_US
dc.subjectCeliteen_US
dc.subjectImmobilizationen_US
dc.subjectLipaseen_US
dc.subjectRicinoleic Aciden_US
dc.subjectCandida-Rugosa Lipaseen_US
dc.subjectHydrolysisen_US
dc.titleProduction of Ricinoleic Acid from Castor Oil by Immobilised Lipasesen_US
dc.typeArticleen_US

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