Characterization, optimization, and in vitro evaluation of cholesterol-free liposomes

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dc.authoridUner, Burcu/0000-0003-4691-0432
dc.authoridErgin, Ahmet Dogan/0000-0002-9387-0085
dc.authorwosidErgin, Ahmet Dogan/AAO-1876-2021
dc.authorwosidUner, Burcu/GPG-1519-2022
dc.contributor.authorErgin, Ahmet Dogan
dc.contributor.authorUner, Burcu
dc.date.accessioned2024-06-12T11:07:41Z
dc.date.available2024-06-12T11:07:41Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjectives: Alzheimer's disease (AD) is a neurological disorder that causes dementia and a progressive loss of thinking, social, and memory abilities. Afterwards, this worsening induces the person incapable of doing even the most fundamental duties. Recent research have proven that Coenzyme-q10 (CoQ10), one of the endogenous fatty acids, suppresses phosphorylated Tau protein which is GM1-ganglioside-bound amyloid beta-protein (GM1-A beta), and inhibiting the formation of amyloid plaques in Alzheimer's disease. Unfortunately, CoQ10 is poorly absorbed due to its high molecular weight (863.34 g/mol) and high lipophilicity, and its bioavailability is quite low. Therefore, we developed the CoQ10-loaded, cholesterol-free liposomes to get across the limitations.Material and methods: Liposomes were developed by ether injection method, and physicochemical characterization of the liposomes were evaluated in terms of particle size, size distribution (PDI), zeta potential, encapsulation efficiency (% EE), and process recovery as well. Release study, DSC analysis, morphological analysis, and cytotoxicity assay were performed with optimized formulations.Results: The particle size, PDI, zeta potential, EE%, and process recovery of the formulations ranged from 343.8 to 167.9 nm; 0.269 to 0.431; (-56) to (-31.7); 18.15-93.48%; 74.63 to 99.62, respectively. According to cytotoxicity tests, liposomes have no significant toxic effect on cells while having decreased p-tau 181 and p-tau 231 proteins (p > 0.05). Conclusions: As a result, the novel cholesterol-free liposome formulation were proved that it might be candidate of including the therapeutical guideline for the future alzheimer's disease treatment with these substantial results.en_US
dc.identifier.doi10.1016/j.jddst.2023.104468
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85153521114en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2023.104468
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22146
dc.identifier.volume84en_US
dc.identifier.wosWOS:000990286800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Drug Delivery Science And Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLiposomeen_US
dc.subjectCoenzyme Q10en_US
dc.subjectCholesterole-Freeen_US
dc.subjectAlzheimeren_US
dc.subjectSH-SY-5Yen_US
dc.subjectSoy Lecithinen_US
dc.subjectEnhanced Solubilityen_US
dc.subjectOxidative Stabilityen_US
dc.subjectElderly-Patientsen_US
dc.subjectDeliveryen_US
dc.subjectBrainen_US
dc.subjectBioavailabilityen_US
dc.subjectNanoparticlesen_US
dc.subjectMemoryen_US
dc.subjectFooden_US
dc.titleCharacterization, optimization, and in vitro evaluation of cholesterol-free liposomesen_US
dc.typeArticleen_US

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