Effects of GnRHa on early embryonic development in mice receiving cyclophosphamide

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dc.contributor.authorKanter, Mehmet
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorSerez, Bilkay
dc.date.accessioned2024-06-12T10:59:06Z
dc.date.available2024-06-12T10:59:06Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThere is a controversy whether GnRH agonist can reduce the deleterious effects of chemotherapy to prevent ovarian failure. We aimed to examine the possible protective effects of a gonadotrophin-releasing hormone agonist (GnRHa) on the fertilization rate and sequential embryonic development in mouse oocytes exposed to Cy. Mice were assigned to three groups of six animals each. A single dose of 75 mg/kg Cy was given intraperitoneally to the Cy mice group. The subcutaneous GnRHa injection was initiated 1 week before and continued for 1 week after the Cy injection in the GnRHa + Cy group. The animals given cyclophosphamide mated 1 week after the Cy injection. At the end of the injection period, the animals underwent a superovulation regime with pregnant mare serum gonadotrophin and human chorionic gonadotrophin and were mated. Early embryos were collected at 48 h after mating. The control group received only the superovulation regime and then mated. Cyclophosphamide caused a significant decrease in the fertilization rate (p < 0.001), whereas the GnRHa improved the rate when compared to control group. The GnRHa induced a marked increase in the rate for 2-cell embryos compared with the Cy group (p = 0.003). In both Cy-injected groups, the rates for the 4-cell embryos were lower than those of the control animals (p < 0.001). However, this rate was higher in the GnRHa + Cy group than in the only Cy group. Morphologically abnormal embryos showed such characteristics as condensed cytoplasm, milky cytoplasm, fragmentation, and an empty zona pellucida. These results demonstrated that the GnRHa preserved the oocyte capability to develop into an embryo against ovarian toxic chemotherapy. Thus, we suggest that GnRHa cotreatment could increase the number and quality of early embryos in mice.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK-107S263]en_US
dc.description.sponsorshipThis work was supported by Scientific and Technological Research Council of Turkey (TUBITAK-107S263). We would like to acknowledge Dr. Imran Kurt Omurlu for the statistical analysis.en_US
dc.identifier.doi10.1007/s00404-015-3831-x
dc.identifier.endpage209en_US
dc.identifier.issn0932-0067
dc.identifier.issn1432-0711
dc.identifier.issue1en_US
dc.identifier.pmid26246413en_US
dc.identifier.scopus2-s2.0-84952865098en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage203en_US
dc.identifier.urihttps://doi.org/10.1007/s00404-015-3831-x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20304
dc.identifier.volume293en_US
dc.identifier.wosWOS:000367603900027en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofArchives Of Gynecology And Obstetricsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCyclophosphamideen_US
dc.subjectGnrh Agonisten_US
dc.subjectMouseen_US
dc.subjectPreimplantation Embryoen_US
dc.subjectInduced Ovarian Damageen_US
dc.subjectReproductive-Performanceen_US
dc.subjectFertility Preservationen_US
dc.subjectHormone Agonisten_US
dc.subjectChemotherapyen_US
dc.subjectMouseen_US
dc.subjectPreventionen_US
dc.subjectTherapyen_US
dc.subjectFemaleen_US
dc.subjectMaturationen_US
dc.titleEffects of GnRHa on early embryonic development in mice receiving cyclophosphamideen_US
dc.typeArticleen_US

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