A comparison of fluorescence and UV-visible spectrometry techniques for thermal phase transitions of agarose gels

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dc.authoridMERGEN, OMER BAHADIR/0000-0002-8829-436X
dc.authoridPEKCAN, Onder/0000-0002-0082-8209
dc.authorwosidMERGEN, OMER BAHADIR/X-6030-2019
dc.authorwosidPEKCAN, Onder/Y-3158-2018
dc.contributor.authorArda, Ertan
dc.contributor.authorKara, Selim
dc.contributor.authorMergen, Omer Bahadir
dc.contributor.authorPekcan, Onder
dc.date.accessioned2024-06-12T10:50:55Z
dc.date.available2024-06-12T10:50:55Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, thermoreversible phase transitions of high (HMP) and low (LMP) melting point agarose gels were investigated by using the UV-vis and fluorescence spectroscopy techniques. Pyranine was added to the aqueous solution of agarose as a fluorescence-probe. Transmitted light (I (tr)) and fluorescence emission (I (fl)) intensities from the gel samples with different agarose concentrations were monitored during the heating (gel-sol) and cooling (sol-gel) processes. For the both techniques, gel-sol (T (gs)) and sol-gel (T (sg)) transition temperatures were determined from the first derivatives of the sigmoidal transition paths. It was observed that the critical transition temperatures obtained from UV-vis and fluorescence data slightly increased depending on the agarose type and concentration, and those values were found to be in accord with each other. Transition activation energies were determined using the Arrhenius type equation and were found to be strongly correlated with the agarose content in the gel system. The produced gel-sol (Delta E (gs)) and sol-gel (Delta E (sg)) transition energies from the fluorescence data were found to be lower than that of the UV-vis data, which was attributed to the temperature-dependent fluorescence quenching effect.en_US
dc.identifier.doi10.1007/s00289-014-1265-4
dc.identifier.endpage175en_US
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84925521980en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage157en_US
dc.identifier.urihttps://doi.org/10.1007/s00289-014-1265-4
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18177
dc.identifier.volume72en_US
dc.identifier.wosWOS:000348982400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPolymer Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgaroseen_US
dc.subjectTransition Energyen_US
dc.subjectCritical Temperatureen_US
dc.subjectFluorescence Probeen_US
dc.subjectThermoreversible Phase Transitionen_US
dc.subjectKappa-Carrageenan Gelsen_US
dc.subjectGelation Mechanismen_US
dc.subjectPhoton Transmissionen_US
dc.subjectCoil-Helixen_US
dc.subjectHydrogelsen_US
dc.subjectModelen_US
dc.subjectHysteresisen_US
dc.subjectNetworksen_US
dc.subjectSystemsen_US
dc.subjectTermsen_US
dc.titleA comparison of fluorescence and UV-visible spectrometry techniques for thermal phase transitions of agarose gelsen_US
dc.typeArticleen_US

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