Immobilization of ?-galactosidase from Kluyveromyces lactis onto polymeric membrane surfaces: Effect of surface characteristics

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dc.contributor.authorGulec, Haci Ali
dc.date.accessioned2024-06-12T11:12:26Z
dc.date.available2024-06-12T11:12:26Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effects of surface characteristics of plain and plasma modified cellulose acetate (CA) membranes on the immobilization yield of beta-galactosidases from Kluyveromyces lactis (KLG) and its galacto-oligosaccharide (GOS) yield, respectively. Low pressure plasma treatments involving oxygen plasma activation, plasma polymerization (PlsP) of ethylenediamine (EDA) and PlsP of 2-mercaptoethanol were used to modify plain CA membrane surfaces. KLG enzyme was immobilized onto plain and oxygen plasma treated membrane surfaces by simple adsorption. Oxygen plasma activation increased the hydrophylicity of CA membrane surfaces and it improved the immobilization yield of the enzyme by 42%. KLG enzyme was also immobilized onto CA membrane surfaces through amino groups created by PlsP of EDA via covalent binding. Plasma action at 60 W plasma power and 15 min. exposure time improved the amount of membrane bounded enzyme by 3.5-fold. The enrichment of the amount of amino groups via polyethyleneimine (PEI) addition enhanced this increase from 3.5-fold to 4.5-fold. Although high enzyme loading was achived (65-83%), both of the methods dramatically decreased the enzyme activity (11-12%) and GOS yield due to probably negative effects of active amino groups. KLG enzyme was more effectively immobilized onto thiolated CA membrane surface created by PlsP of 2-mercaptoethanol with high immobilization yield (70%) and especially high enzyme activity (46%). Immobilized enzymes on the CA membranes treated by PlsP were successively reutilized for 5-8 cycles at 25 degrees C and enzymatic derivatives retained approximately 75-80% of their initial activites at the end of the reactions. (c) 2012 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipFund of Scientific Research Projects of Trakya University (TUBAP) [2011/142]en_US
dc.description.sponsorshipThis study was supported in part by The Fund of Scientific Research Projects of Trakya University (TUBAP; project no:2011/142).en_US
dc.identifier.doi10.1016/j.colsurfb.2012.11.039
dc.identifier.endpage90en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid23298592en_US
dc.identifier.scopus2-s2.0-84872140102en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage83en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2012.11.039
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23177
dc.identifier.volume104en_US
dc.identifier.wosWOS:000316645100013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofColloids And Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlasma Treatmenten_US
dc.subjectMembraneen_US
dc.subjectImmobilizationen_US
dc.subjectBeta-Galactosidaseen_US
dc.subjectKluyveromyces Lactisen_US
dc.subjectFooden_US
dc.subjectCellulose-Acetate Membraneen_US
dc.subjectCovalent Immobilizationen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectLactose Hydrolysisen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectInvertaseen_US
dc.subjectOligosaccharidesen_US
dc.subjectPolyethyleneimineen_US
dc.subjectPolyanilineen_US
dc.subjectTemperatureen_US
dc.titleImmobilization of ?-galactosidase from Kluyveromyces lactis onto polymeric membrane surfaces: Effect of surface characteristicsen_US
dc.typeArticleen_US

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