The false-positive responses of analgesic drugs to the intradermal serotonin- and compound 48/80-induced scratches as an animal model of itch

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dc.authoridUlugol, Ahmet/0000-0003-4643-1124
dc.authoridAkar Karadayı, Aysegul/0000-0002-9908-6988
dc.authoridGunaydin, Caner/0000-0002-8304-832X
dc.authoridGunduz, Ozgur/0000-0002-2470-3021
dc.authorwosidGunaydin, Caner/A-9108-2018
dc.authorwosidUlugol, Ahmet/V-9665-2019
dc.authorwosidAkar Karadayı, Aysegul/A-5981-2018
dc.authorwosidGunaydin, Caner/AAP-2515-2020
dc.authorwosidGÜNDÜZ, Özgür/AAH-8717-2019
dc.authorwosidGunduz, Ozgur/A-2351-2016
dc.contributor.authorIlkaya, Fatih
dc.contributor.authorYesilyurt, Ozgur
dc.contributor.authorSeyrek, Melik
dc.contributor.authorGunduz, Ozgur
dc.contributor.authorIde, Tayfun
dc.contributor.authorAkar, Ahmet
dc.contributor.authorUlugol, Ahmet
dc.date.accessioned2024-06-12T10:51:36Z
dc.date.available2024-06-12T10:51:36Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIntradermal injection of pruritogens such as serotonin, histamine and compound 48/80 into the skin and then, the evaluation of the scratching behavior is the commonly used animal model to advance pruritic research and drug development. However, predictive validity of this model is poorly documented. There is a close interaction between itch and pain sensations with regard to mediation through an anatomically and functionally identical neuronal pathway. One approach is whether the existing animal model of itch differentiates itch or pain to show efficacy of clinically effective analgesic drugs as a back translation. In this study, we explored the effects of different group of analgesic drugs on serotonin and compound 48/80-induced scratching behavior in Balb-C mice. Serotonin (25 mu g) and compound 48/80 (100 mu g) was injected intradermally in a volume of 50 mu l into the rostra! part of skin on the back of male mice and scratches were counted for a 30-min observation period. Morphine (1, 3, 10 mg/kg), tramadol (20, 40, 80 mg/kg), cannabinoid agonist CP 55,940 (0.1, 0.3, 1 mg/kg), paracetamol (100, 200, 300 mg/kg) and diclofenac (50, 100, 200 mg/kg) were given intraperitoneally 30 min prior to pruritogen injection. The analgesic drugs dose dependently blocked serotonin and compound 48/80-induced straching behavior with exerting complete inhibition at certain doses. Our data suggests that intradermal pruritogen-induced scratching models may not discriminate pain and itch sensations and give false positive results when standard analgesic drugs are used.en_US
dc.identifier.doi10.21307/ane-2017-023
dc.identifier.endpage243en_US
dc.identifier.issn0065-1400
dc.identifier.issn1689-0035
dc.identifier.issue3en_US
dc.identifier.pmid27685776en_US
dc.identifier.scopus2-s2.0-84988884564en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage234en_US
dc.identifier.urihttps://doi.org/10.21307/ane-2017-023
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18408
dc.identifier.volume76en_US
dc.identifier.wosWOS:000385338800008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNencki Inst Experimental Biologyen_US
dc.relation.ispartofActa Neurobiologiae Experimentalisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnalgesic Drugsen_US
dc.subjectItchen_US
dc.subjectScratchingen_US
dc.subjectPruritogenen_US
dc.subjectNeck Modelen_US
dc.subjectFalse Positiveen_US
dc.subjectEndocannabinoid Modulationen_US
dc.subjectProstaglandin E-2en_US
dc.subjectReceptor Agonisten_US
dc.subjectDouble-Blinden_US
dc.subjectPainen_US
dc.subjectPruritusen_US
dc.subjectMiceen_US
dc.subjectBehavioren_US
dc.subjectMorphineen_US
dc.subjectSkinen_US
dc.titleThe false-positive responses of analgesic drugs to the intradermal serotonin- and compound 48/80-induced scratches as an animal model of itchen_US
dc.typeArticleen_US

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