Scanning of some herbal tissues to be used with biosensors as polyphenol oxidase enzyme source

Basit Öğe Kaydı
dc.contributor.authorSağıroğlu, Ayten
dc.contributor.authorÖzcan, Hakkı Mevlüt
dc.contributor.authorHasancebeci, Özhan
dc.date.accessioned2024-06-12T10:16:15Z
dc.date.available2024-06-12T10:16:15Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe purpose of this study is to examine levels of polyphenol oxidase (PPO) activities in the crude extracts of seventeen different herbal tissues (Banana peel, Jerusalem artichoke, black grape, buckthorn, fresh bean, pea, cactus apple, aloe vera, cabbage turnip graim, cabbage turnip vegetable, celeriac, quince, artichoke, aubergine, Trabzon palm, dry carob) with spectrophotometric method. The three herbal tissues (Anamur banana peel, Jerusalem artichoke and fresh broad bean) were found to have higher PPO activities than other tissues, 638, 3360, 3677 U/min.mg protein, respectively. Biologic oxygen biosensors were prepared by cross-linked thin film immobilization method, in the presence of gelatin and glutaraldehyde, with three herbal tissues used as biomaterials and these biosensors were used for detection of phenolic compounds via determining the concentration of consumed oxygen throughout the reaction medium. The typical calibration curves for the Anamur banana peel, Jerusalem artichoke and fresh broad bean based sensors revealed a linear range of 5-40 μM, 2-10 μM, 10-100 μM, respectively. In reproducibility studies, variation coefficiets (CV) and standard deviations (SD) were calculated as 1.4%-0.3294 μM, 2%-0.8392 μM and 1%-0.0746 μM, respectively.en_US
dc.identifier.endpage312en_US
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue4en_US
dc.identifier.startpage303en_US
dc.identifier.trdizinid103843en_US]
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/103843
dc.identifier.urihttps://hdl.handle.net/20.500.14551/15149
dc.identifier.volume37en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHacettepe Journal of Biology and Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleScanning of some herbal tissues to be used with biosensors as polyphenol oxidase enzyme sourceen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu