Effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cells

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dc.contributor.authorEskiocak, Sevgi
dc.contributor.authorÖzgün, Eray
dc.contributor.authorÖzgün, Gülben Sayılan
dc.contributor.authorTabakçıoğlu, Kıymet
dc.contributor.authorGökmen, Selma Süer
dc.date.accessioned2024-06-12T10:04:37Z
dc.date.available2024-06-12T10:04:37Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAim: Palmitate is one of the most abundant fatty acid in both liver of healthy individuals and in patients with non-alcoholic fatty liver disease. Palmitate-induced steatosis in HepG2 cells is an in vitro non-alcoholic fatty liver disease model to investigate acute harmful effects of fat overaccumulation in the liver. Non-alcoholic fatty liver disease is strongly associated with atherosclerosis. Paraoxonase-1 and paraoxonase-3 are anti-atherosclerotic enzymes which are bound to high density lipoprotein in circulation and they are primarily synthesized by liver. There is no study that investigated the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes. The aim of present study was to investigate the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in HepG2 cells. Methods: To induce steatosis, cells were incubated with 0.4, 0.7 and 1 mM palmitate for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Cells were stained with oil red O and triglyceride levels were measured. Paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting, their mRNA expression were measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. Results: All palmitate concentrations caused a significant increase on paraoxonase-1 mRNA levels. Palmitate concentrations did not cause a significant change on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA levels and arylesterase activities. Conclusion: Our study showed that palmitate-induced steatosis up-regulates paraoxonase-1 mRNA, has no effect on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA and arylesterase activity in HepG2 cells.en_US
dc.identifier.doi10.25000/acem.623975
dc.identifier.endpage147en_US
dc.identifier.issn2564-6567
dc.identifier.issue3en_US
dc.identifier.startpage142en_US
dc.identifier.trdizinid319440en_US]
dc.identifier.urihttps://doi.org/10.25000/acem.623975
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/319440
dc.identifier.urihttps://hdl.handle.net/20.500.14551/12924
dc.identifier.volume4en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofArchives of Clinical and Experimental Medicineen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleEffect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in human-derived liver (HepG2) cellsen_US
dc.typeArticleen_US

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