Preparation of new molecularly imprinted quartz crystal microbalance hybride sensor system for 8-hydroxy-2?-deoxyguanosine determination

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dc.authoridÖzcan, Ayça Atılır/0000-0003-4402-6280
dc.authoridErsoz, Arzu/0000-0002-2726-2065
dc.authorwosidatilir ozcan, ayca/AID-6411-2022
dc.authorwosidÖzcan, Ayça Atılır/C-7915-2019
dc.authorwosidDenizli, Adil/G-5151-2012
dc.contributor.authorSay, Ridvan
dc.contributor.authorGultekin, Aytac
dc.contributor.authorOzcan, Ayca Atilir
dc.contributor.authorDenizli, Adil
dc.contributor.authorErsoz, Arzu
dc.date.accessioned2024-06-12T11:08:41Z
dc.date.available2024-06-12T11:08:41Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe routine measurement of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in biological samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. A new 8-OHdG imprinted quartz crystal microbalance (QCM) sensor has been developed for selective determination of 8-OHdG in serum samples. To fulfil the desired results, we have used methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] and methacryloyl histidine-Pt(II) [MAH-Pt(II)] as metal-chelating monomers via double metal coordination-chelation interactions for the preparation of additional selective molecular imprinted polymers (MIP). The study includes the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM chip. The obtained results have showed that the application of double metal-chelate monomer systems has been more effective than single metal-chelate monomer systems. In this study, the detection limit and the linear working range were found to be 0.0075 and 0.0100-3.5 mu M, respectively. The affinity constant (K-affinity) was found to be 1.54 x 10(5) M-1 for 8-OHdG using MAH-Pt-8-OHdG-MAAP-Fe based thin film. Also, selectivity of prepared QCM sensor was found as being very high in the presence of competitive species. At the last step of this procedure, 8-OHdG level in blood serum which belongs to a intestinal cancer patient was determined by the prepared QCM sensor. (c) 2009 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.aca.2009.03.022
dc.identifier.endpage86en_US
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.issue1-2en_US
dc.identifier.pmid19362624en_US
dc.identifier.scopus2-s2.0-64049084288en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage82en_US
dc.identifier.urihttps://doi.org/10.1016/j.aca.2009.03.022
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22524
dc.identifier.volume640en_US
dc.identifier.wosWOS:000265763500012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMolecular Imprinted Polymersen_US
dc.subjectQuartz Crystal Microbalanceen_US
dc.subject8-Hydroxy-2 '-Deoxyguanosineen_US
dc.subjectMetal-Chelate Interactionen_US
dc.subjectOxidative Dna-Damageen_US
dc.subjectSubstrate-Selective Polymersen_US
dc.subjectLiquid-Chromatographyen_US
dc.subject8-Hydroxydeoxyguanosineen_US
dc.subjectRecognitionen_US
dc.subjectImmunosensoren_US
dc.subjectRemovalen_US
dc.subjectMarkeren_US
dc.subjectUrineen_US
dc.titlePreparation of new molecularly imprinted quartz crystal microbalance hybride sensor system for 8-hydroxy-2?-deoxyguanosine determinationen_US
dc.typeArticleen_US

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