An immunohistochemical analysis of the neuroprotective effects of memantine, hyperbaric oxygen therapy, and brimonidine after acute ischemia reperfusion injury

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dc.authoridUSLU, UNAL/0000-0003-3953-7131
dc.authoridYiğit, Ulviye/0000-0003-0176-1509
dc.authoridCumbul, Alev/0000-0002-9491-8220
dc.authorwosidUSLU, UNAL/H-1226-2011
dc.authorwosidYiğit, Ulviye/ABD-9562-2021
dc.authorwosidCUMBUL, Alev/H-3991-2011
dc.authorwosidAktaş, Şamil/AAD-6656-2020
dc.contributor.authorYigit, Ulviye
dc.contributor.authorErdenoz, Serkan
dc.contributor.authorUslu, Unal
dc.contributor.authorOba, Ersin
dc.contributor.authorCumbul, Alev
dc.contributor.authorCagatay, Halil
dc.contributor.authorAktas, Samil
dc.date.accessioned2024-06-12T11:19:48Z
dc.date.available2024-06-12T11:19:48Z
dc.date.issued2011
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: This study applies treatment methods to rat retinas subjected to acute ischemia reperfusion injury and compares the efficacy of memantine, hyperbaric oxygen (HBO) therapy, and brimonidine by histopathological examination. Methods: Thirty adult Wistar albino rats were divided into five groups after retinal ischemia was induced by elevating the intraocular pressure to 120 mmHg. The groups were as follows: group 1: control; group 2: acute retinal ischemia (ARI) model but without treatment group; group 3: memantine (MEM) treatment group; group 4: HBO therapy group; and group 5: brimonidine treatment (BRI) group. In the control group, right eyes were cannulated with a 30-gauge needle and removed without causing any intraocular pressure change. The ARI group was an acute retinal ischemia model, but without treatment. In the MEM group, animals were given a unique dose of intravenous 25 mg/kg memantine by the tail vein route after inducing ARI. In the HBO group, at 2 h following ARI, HBO treatment was applied for nine days. In the BRI group, a 0.15% brimonidine tartrate eye drop treatment was applied twice a day (BID) for seven days before ARI. Twenty-one days after establishing ischemia reperfusion, the right eyes were enucleated after the cardiac gluteraldehyde perfusion method, and then submitted to histological evaluation. Results: On average, the total retinal ganglion cell number was 239.93 +/- 8.60 in the control group, 125.14 +/- 7.18 in the ARI group, 215.89 +/- 8.36 in the MEM group, 208.69 +/- 2.05 in the HBO group, and 172.27 +/- 8.16 in the BRI group. Mean apoptotic indexes in the groups were 1.1 +/- 0.35%, 57.71 +/- 0.58%, 23.57 +/- 1.73%, 15.63 +/- 0.58%, and 29.37 +/- 2.55%, respectively. Conclusions: The present study shows that memantine, HBO, and brimonidine therapies were effective in reducing the damage induced by acute ischemia reperfusion in the rat retina. Our study suggests that these treatments had beneficial effects due to neuroprotection, and therefore may be applied in clinical practice.en_US
dc.identifier.endpage1033en_US
dc.identifier.issn1090-0535
dc.identifier.issue115en_US
dc.identifier.pmid21541269en_US
dc.identifier.scopus2-s2.0-79955578772en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1024en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25353
dc.identifier.volume17en_US
dc.identifier.wosWOS:000289944700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMolecular Visionen_US
dc.relation.ispartofMolecular Visionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRetinal Artery-Occlusionen_US
dc.subjectGanglion-Cell Deathen_US
dc.subjectPerfusion-Fixationen_US
dc.subjectFactor Expressionen_US
dc.subjectMacular Edemaen_US
dc.subjectRat Modelen_US
dc.subjectBrainen_US
dc.subjectAntagonisten_US
dc.subjectMechanismsen_US
dc.subjectProtectionen_US
dc.titleAn immunohistochemical analysis of the neuroprotective effects of memantine, hyperbaric oxygen therapy, and brimonidine after acute ischemia reperfusion injuryen_US
dc.typeArticleen_US

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