Growth Arrest-Specific 6 (Gas6) and TAM Receptors in Mouse Platelets

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dc.authoridOzakpınar, Ozlem Bingol/0000-0003-0287-5639;
dc.authorwosidOzakpınar, Ozlem Bingol/ABI-4144-2020
dc.authorwosidOzakpinar, Ozlem Bingol/ACB-1160-2022
dc.contributor.authorUras, Fikriye
dc.contributor.authorKucuk, Burhanettin
dc.contributor.authorOzakpinar, Ozlem Bingol
dc.contributor.authorDemir, Ahmet Muzaffer
dc.date.accessioned2024-06-12T10:54:53Z
dc.date.available2024-06-12T10:54:53Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: Growth arrest-specific 6 (Gas6) is a newly discovered vitamin K-dependent protein, which is a ligand for TAM receptors [Tyro3 (Sky), Axl, and Mer] from the tyrosine kinase family. Gas6 knockout mice were resistant to venous and arterial thrombosis. There are contradictory reports on the presence of Gas6 and its receptors in mouse platelets. The objective of this study was to investigate whether Gas6 and its receptors were present in mouse platelets or not. Materials and Methods: Specific pathogen-free BALB/c male and female mice of 8-10 weeks old and 25-30 g in weight were anesthetized under light ether anesthesia and blood samples were taken from their hearts. RNAs were isolated from isolated platelets, and then mRNAs encoding Gas6 and TAM receptors were detected by reverse transcription-polymerase chain reaction (RT-PCR). Protein concentrations of Gas6 and TAM receptors in platelets were measured by ELISA, but not those of Mer, because of the absence of any commercial ELISA kit for mouse specimens. Results: RT-PCR results indicated the presence of mRNAs encoding Gas6 and Mer in mouse platelets. However, although RTPCR reactions were performed at various temperatures and cycles, we could not detect the presence of mRNAs encoding Axl and Tyro3 (Sky). Receptor protein levels of Axl and Tyro3 were below the detection limits of the ELISA method. Conclusion: We found the presence of mRNAs encoding Gas6 and the receptor Mer in mouse platelets, but not Axl and Tyro3. Gas6, Axl, and Tyro3 protein levels were below the detection limits of the ELISA. The presence of mRNA is not obvious evidence of protein expression in platelets that have no nucleus or DNA. Further studies are required to clarify the presence of Gas6/TAM receptors in platelets using real-time PCR and more sensitive immunological methods, and future studies on mechanisms will indicate whether the Gas6/TAM pathway is a strategy for treatment of disorders.en_US
dc.identifier.doi10.4274/tjh.2013.0097
dc.identifier.endpage63en_US
dc.identifier.issn1300-7777
dc.identifier.issn1308-5263
dc.identifier.issue1en_US
dc.identifier.pmid25805676en_US
dc.identifier.scopus2-s2.0-84924546617en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage58en_US
dc.identifier.trdizinid179365en_US
dc.identifier.urihttps://doi.org/10.4274/tjh.2013.0097
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/179365
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19200
dc.identifier.volume32en_US
dc.identifier.wosWOS:000350900200008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Yayinciliken_US
dc.relation.ispartofTurkish Journal Of Hematologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBlood Plateletsen_US
dc.subjectGrowth Arrest-Specific Protein 6en_US
dc.subjectHemostasisen_US
dc.subjectVascular Smooth-Muscleen_US
dc.subjectTyrosine Kinaseen_US
dc.subjectProtein-Sen_US
dc.subjectExpression Analysisen_US
dc.subjectBlood-Coagulationen_US
dc.subjectEndothelial-Cellsen_US
dc.subjectAxlen_US
dc.subjectGeneen_US
dc.subjectMeren_US
dc.subjectLiganden_US
dc.titleGrowth Arrest-Specific 6 (Gas6) and TAM Receptors in Mouse Plateletsen_US
dc.typeArticleen_US

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