Some biochemical properties of polyphenol oxidase from celery

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dc.contributor.authorYagar, H
dc.date.accessioned2024-06-12T11:12:35Z
dc.date.available2024-06-12T11:12:35Z
dc.date.issued2004
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPolyphenol oxidase (PPO, EC 1.14.18.1) was extracted from celery roots (Apium graveolens L.) with 0.1 M phosphate buffer, pH 7.0. The PPO was partially purified by (NH4)(2)SO4 and dialysis. Substrate specificity experiments were carried out with catechol, pyrogallol, L-DOPA, p-cresol, resorcinol, and tyrosine. The Km for pyrogallol, catechol, and L-DOPA were 4.5, 8.3, and 6.2 mM, respectively, at 25degreesC. Data for V-max/K-m values, which represent catalytic efficiency, show that pyrogallol has the highest value. The optimum pH and temperature were determined with catechol, pyrogallol, and L-DOPA. Optimum pH was 7.0 for catechol and L-DOPA, and 7.5 for pyrogallol. Optimum temperatures for maximum PPO activity were 25degreesC for pyrogallol, 40degreesC for catechol, and 45degreesC for L-DOPA. Heat inactivation studies showed a decrease in enzymatic activity at temperatures above 60degreesC. The order of inhibitor effectiveness was: L-cysteine > ascorbic acid > glycine > resorcinol > NaCl.en_US
dc.identifier.doi10.1081/PB-200031054
dc.identifier.endpage397en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue4en_US
dc.identifier.pmid15553907en_US
dc.identifier.scopus2-s2.0-7244223402en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage387en_US
dc.identifier.urihttps://doi.org/10.1081/PB-200031054
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23227
dc.identifier.volume34en_US
dc.identifier.wosWOS:000224778200007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolyphenol Oxidaseen_US
dc.subjectCeleryen_US
dc.subjectApium Graveolensen_US
dc.subjectIsolationen_US
dc.subjectCharacterisationen_US
dc.subjectPyrus-Communis Len_US
dc.subjectPurificationen_US
dc.subjectAvocadoen_US
dc.subjectBananaen_US
dc.subjectPearsen_US
dc.titleSome biochemical properties of polyphenol oxidase from celeryen_US
dc.typeArticleen_US

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