Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage

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dc.authoriddelen, ozlem/0000-0001-5652-2658
dc.authoridUz, Yesim/0000-0002-0381-4590
dc.contributor.authorDelen, Ozlem
dc.contributor.authorUz, Yesim H.
dc.date.accessioned2024-06-12T11:08:26Z
dc.date.available2024-06-12T11:08:26Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of the study was to investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) against methotrexate (MTX)-induced testicular damage in rats. Forty Wistar albino male rats were divided into equally four groups: Control group (saline solution, IP), PDTC group (100 mg/kg PDTC,IP, 10 days), MTX group (20 mg/kg MTX, IP, single dose, on the 6th day) and MTX + PDTC group (100 mg/kg PDTC, IP, 10 days and 20 mg/kg MTX, IP, single dose, on the 6th day). After 10 days, testicular tissues were excised for morphometric, histological and immunohistochemical evaluations. Serum testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and prokineticin 2 (PK2) levels were determined. Body and testicular weights were measured. Testicular damage was assessed by histological evaluation. Nuclear factor kappa B (NFkB), nuclear factor erythroid 2 related factor 2 (Nrf2) and PK2 immunoreactivities were evaluated by HSCORE. Body and testicular weights, serum FSH, LH, testosterone levels, seminiferous tubule diameter and germinal epithelial thickness were significantly decreased in the MTX group. However, serum PK2 level, histologically damaged seminiferous tubules and interstitial field width were significantly increased. Additionally, there was an increase in NFkB and PK2 immunoreactivity, whereas there was a significant decrease in Nrf2 immunoreactivity. PDTC significantly improved hormonal, morphometric, histological and immunohistochemical findings. Taken together, we conclude that PDTC may reduce MTX-induced testicular damage via NFkB, Nrf2 and PK2 signaling pathways.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Trakya University [TUBAP 2018/103]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Scientific Research Projects Coordination Unit of Trakya University (TUBAP 2018/103).en_US
dc.identifier.doi10.1177/09603271211035674
dc.identifier.endpageS177en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue12_SUPPLen_US
dc.identifier.pmid34340576en_US
dc.identifier.scopus2-s2.0-85112072355en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpageS164en_US
dc.identifier.urihttps://doi.org/10.1177/09603271211035674
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22435
dc.identifier.volume40en_US
dc.identifier.wosWOS:000683112500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMethotrexateen_US
dc.subjectNuclear Factor Erythroid 2 Related Factor 2en_US
dc.subjectNuclear Factor Kappa Ben_US
dc.subjectProkineticin 2en_US
dc.subjectPyrrolidine Dithiocarbamateen_US
dc.subjectTestisen_US
dc.subjectFactor-Kappa-Ben_US
dc.subjectInduced Testis Injuryen_US
dc.subjectAlpha-Lipoic Aciden_US
dc.subjectOxidative Stressen_US
dc.subjectMale-Infertilityen_US
dc.subjectProkineticin 2en_US
dc.subjectToxicityen_US
dc.subjectActivationen_US
dc.subjectInhibitorsen_US
dc.subjectNephrotoxicityen_US
dc.titleProtective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damageen_US
dc.typeArticleen_US

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