Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage

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dc.authoridUlucam, Enis/0000-0002-4686-7350
dc.authoridCerkezkayabekir, Aysegul/0000-0001-5537-1042
dc.authoridSanal, Filiz/0000-0002-5830-4811;
dc.authorwosidUlucam, Enis/AAG-9204-2019
dc.authorwosidKaraoz, Erdal/AAO-1470-2020
dc.authorwosidCerkezkayabekir, Aysegul/Q-6629-2018
dc.authorwosidSanal, Filiz/O-9428-2019
dc.authorwosidBAKAR, Elvan/AAE-9247-2022
dc.authorwosidUluçam, Enis/HQZ-3831-2023
dc.contributor.authorInan, M.
dc.contributor.authorBakar, E.
dc.contributor.authorCerkezkayabekir, A.
dc.contributor.authorSanal, F.
dc.contributor.authorUlucam, E.
dc.contributor.authorSubasi, C.
dc.contributor.authorKaraoz, E.
dc.date.accessioned2024-06-12T11:08:23Z
dc.date.available2024-06-12T11:08:23Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Mesenchymal stemcells (MSCs) may have beneficial effects in reversing intestinal damage resulting fromcirculatory disorders. The hypothesis of this study is thatMSCs increase antioxidant capacity of small bowel tissue following intestinal ischemia reperfusion (I/R) damage. Methods: A total of 100 rats were used for the control group and three experimental groups, as follows: the sham control, local MSC, and systemic MSC groups. Each group consisted of 10 animals on days 1, 4, and 7 of the experiment. Ischemiawas established by clamping the superior mesenteric artery (SMA) for 45min; following this, reperfusion was carried out for 1, 4, and 7 days in all groups. In the local and systemic groups, MSCs were administered intravenously and locally just after the ischemia, and they were investigated after 1, 4, and 7 days. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) activities, as well as malondialdehyde (MDA) and total protein levels, were measured. Histopathological analysis was performed using light and electron microscopy. The indicators of proliferation from the effects of anti-and pro-inflammatory cytokines were evaluated using immunohistochemistry. Results: MDA was increased (P < 0.05) in the sham control group and decreased (P < 0.05) in the MSC groups. SOD, CAT, and Gpx were decreased in the localMSC group (P < 0.05). The highest level of amelioration was observed on day 7 in the local MSC group via light and electron microscopy. It was found that the MSCs arrived at the damaged intestinal wall in the MSC groups immediately after injection. Pro-inflammatory cytokines interleukin-1 beta(IL1 beta), transforming growth factor-beta 1 (TGF beta 1), tumor necrosis factor-alpha (TNF alpha), IL6, MIP2, and MPO decreased (P < 0.05), while anti-inflammatory cytokines EP3 and IL1ra increased (p < 0.05) in the local and systemic MSC groups. In addition, proliferation indicators, such as PCNA and KI67, increased (P < 0.05) in the local and systemic MSC groups. Conclusions: Parallel to our hypothesis, MSC increases the antioxidant capacity of small bowel tissue after intestinal I/R damage. The MSCs migrated to the reperfused small intestine by homing and reduced oxidative stress via the effects of SOD, CAT, and Gpx, as well as reducing the MDA level; thus, they could increase antioxidant capacity of intestine and have a therapeutic effect on the damaged tissue. We think that this effectwas achieved via scavenging of oxygen radicals, suppression of pro-inflammatory cytokines, and increasing the expression of anti-inflammatory cytokines. (C) 2017 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112S124]en_US
dc.description.sponsorshipThe authorswould like to thank the Scientific and Technological Research Council of Turkey (TUBITAK), as this project was supported by grants from this institution (no. 112S124). The authors would also like to thank the staff of KOGEM, Kocaeli, Turkey, for their support in the isolation, characterization, identification, and monitoring of MSCs, as well as the preparation of histological sections; the Electron Microscope Laboratory of Osmangazi University, Eskisehir, Turkey, for preparation of electron microscopic sections; and the Experimental Animal Breeding and Research Unit of Trakya University, Edirne, Turkey for the care and preparation of rats for the experimental surgical procedure.en_US
dc.identifier.doi10.1016/j.jpedsurg.2016.12.024
dc.identifier.endpage1206en_US
dc.identifier.issn0022-3468
dc.identifier.issn1531-5037
dc.identifier.issue7en_US
dc.identifier.pmid28118930en_US
dc.identifier.scopus2-s2.0-85009957990en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1196en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpedsurg.2016.12.024
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22397
dc.identifier.volume52en_US
dc.identifier.wosWOS:000405362500025en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMesenchymal Stem Cellen_US
dc.subjectIschemiaen_US
dc.subjectReperfusionen_US
dc.subjectSmall Intestineen_US
dc.subjectAntioxidant Capacityen_US
dc.subjectLocal And Systemic Administrationen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectGlutathione-Peroxidaseen_US
dc.subjectSuperoxide-Dismutaseen_US
dc.subjectLung Injuryen_US
dc.subjectLesionen_US
dc.subjectLiveren_US
dc.titleMesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damageen_US
dc.typeArticleen_US

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