Effects of scrotal hyperthermia on Leydig cells in long-term: a histological, immunohistochemical and ultrastructural study in rats

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dc.authorwosidAktas, Cevat/D-8468-2011
dc.contributor.authorKanter, Mehmet
dc.contributor.authorAktas, Cevat
dc.date.accessioned2024-06-12T11:03:42Z
dc.date.available2024-06-12T11:03:42Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractWe have previously demonstrated that scrotal hyperthermia induce Leydig cell (LC) damage in short-term. The objectives of this pilot study were to investigate morphological changes and regulation of steroidogenesis on LC in long-term and the time of observation were extended to investigate whether the LC would eventually make a recovery after scrotal hyperthermia. The rats were randomly allotted into one of four groups: A (control), B (70 days after scrotal hyperthermia), C (105 days after scrotal hyperthermia), D (140 days after scrotal hyperthermia); each group contain seven animals. Scrotal hyperthermia was carried out in a thermostatically controlled water bath at 43A degrees C for 30 min once daily for six consecutive days. Control rats were treated in the same way, except the testes were immersed in a water bath maintained at 22A degrees C. Hyperthermia applied rats were sacrificed under 50 mg/kg ketamine anaesthesia after 70, 105 and 140 days, and biopsy materials of testes were obtained for light and electron microscopic examinations. Morphologically normal and the number of testosterone positive LC was significantly higher in 140 days after last heat than all other heat treatment groups. In heat treated groups, a dilated smooth endoplasmic reticulum, swollen mitochondria, and vanished mitochondrial cristae were observed. In the 140 days after scrotal hyperthermia, the severities of degenerative changes of LC were less than that observed in the other heat treated groups. We conclude that, scrotal hyperthermia cause morphological damaging and impaired steroidogenesis in LC and recovery of these findings were noted first time in 140 days after the last heat treatment.en_US
dc.identifier.doi10.1007/s10735-009-9222-5
dc.identifier.endpage130en_US
dc.identifier.issn1567-2379
dc.identifier.issue2en_US
dc.identifier.pmid19484498en_US
dc.identifier.scopus2-s2.0-67651229219en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage123en_US
dc.identifier.urihttps://doi.org/10.1007/s10735-009-9222-5
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21761
dc.identifier.volume40en_US
dc.identifier.wosWOS:000267784900005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Molecular Histologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmunohistochemistryen_US
dc.subjectUltrastructureen_US
dc.subjectLeydig Cellen_US
dc.subjectScrotal Hyperthermiaen_US
dc.subjectRaten_US
dc.subjectAcute Regulatory Proteinen_US
dc.subjectExperimental Cryptorchidismen_US
dc.subjectSemen Qualityen_US
dc.subjectInfertile Menen_US
dc.subjectBlood-Flowen_US
dc.subjectMale-Miceen_US
dc.subjectIn-Vivoen_US
dc.subjectHeaten_US
dc.subjectTestisen_US
dc.subjectSpermatogenesisen_US
dc.titleEffects of scrotal hyperthermia on Leydig cells in long-term: a histological, immunohistochemical and ultrastructural study in ratsen_US
dc.typeArticleen_US

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